tag:blogger.com,1999:blog-43717448331867813902024-03-12T16:50:02.517-07:00Photosyn ThisJ.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.comBlogger18125tag:blogger.com,1999:blog-4371744833186781390.post-63877429506611407352014-07-09T15:15:00.000-07:002014-09-17T06:40:08.832-07:00Let's buy spinach!!!!<div dir="ltr" style="text-align: left;" trbidi="on">
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Really quick blog post about the purchasing of spinach for my experiments. The key place to go is the market just down the road from Ruban's Lab, Whitechapel market. The reason why we prefer to purchase market spinach compared to supermarket is often the fact that spinach in the market appears more leafy and rich in a mossy green colour, rich mossy green = rich in chloroplast, exactly what we need for this experiment! </div>
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Whitechapel, for those who don't know it is a vibrant, very vivid, diverse part of London. It is the home to Queen Mary's, Bart's and the London Medical & Dental school, it's a place where I sometimes find myself due to lectures. Anyways, this area had a huge migration of Bangladeshi migrants back in the day, so this area has a huge influence with Bangladeshi culture and various of these market stalls are ran my people of Bangladeshi decent. Here are a few pictures, pardon me these pictures are quite terrible, as I tried to take photos without making it obvious that I was actually taking photos:</div>
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Yeah an ordinary main road with market stalls running along the sides.</div>
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The only problem I have of this market is that most of the stalls don't stock spinach throughout the day and there are set times which spinach is actually available. When the actual availability time for spinach is, no one ever knows exactly, so sometimes you ask for spinach, the stall vendor says an hour, you come back in an hour and there is still no spinach....It's basically playing the waiting game for spinach.</div>
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J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-10764306597059140292014-07-07T19:30:00.000-07:002014-09-17T06:17:54.817-07:00Start Week 7: Buffer Prep<div dir="ltr" style="text-align: left;" trbidi="on">
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Starting the week preparing my own set of BBY buffers. Oh the joy! *sarcasm*<br />
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Don't get me wrong, I do enjoy the lab but preparing buffers for your actual protocol can be quite mundane. The whole process is essentially weighing and measuring accurately the certain amount of chemicals you need in your buffers etc. A process you compare to actually conducting BBY or results analysis, just doesn't compare with the excitement driven adrenaline levels. Nevertheless, this whole process of buffer prep is crucial to the success of the actual protocol- to yield the desired results as predicted. So no matter how mundane this task may appear to be, disarray the entire buffer preparation and goodbye to your hard work you may spend on your protocol. Every gram, pH set for the buffer could honestly severely disrupt your results.<br />
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In short words, every single step matters in science.<br />
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Sometimes you may be lucky and a mistake could actually lead to a publication! However, only the ones who are prepared will ever find a success ridden mistake! One of my favourite quotes, clearly portraying such matter belongs to the wise words spoken by Louis Pasteur. Louis Pasteur being famously known for his discovery of vaccination~~~<br />
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<tr><td class="tr-caption" style="text-align: center;">True, wise words<span style="background-color: white; color: #222222; font-family: arial, sans-serif; font-size: 16px; line-height: 19.2000007629395px; text-align: left;">❤ </span></td></tr>
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<u>Anyways here are some pictures from the day:</u><br />
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<tr><td class="tr-caption" style="text-align: center;">Following the instructions for buffer prep. My nice little printed out booklet stuck into my lab book, with the always needed concentration x volume calculation equations !</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj3UW0Gg50VAMufgn4HCClm8fWofxX-LA8zmzu5lHazCVupFZKCoJilw2U0lxJKirZYI4Yy849LuBzUPE06yZPapIMiR58btRxLGd7o6Wjw84NsQcwalnf4_XgLCGLJ8SX9-W8ayLZ2N56y/s1600/IMG_20140709_131421.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj3UW0Gg50VAMufgn4HCClm8fWofxX-LA8zmzu5lHazCVupFZKCoJilw2U0lxJKirZYI4Yy849LuBzUPE06yZPapIMiR58btRxLGd7o6Wjw84NsQcwalnf4_XgLCGLJ8SX9-W8ayLZ2N56y/s1600/IMG_20140709_131421.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Cleaning the balance as indicated with the sign, the paint bush really gets to all the nook and cranny</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi858DMtKrz-OWrOtcSNoy8ThriPTHlJVRzPXugXslRSiAoRRI9V5Ww7iaYXKFh49hqimjh_tohzFNNL1l7oB_gwu-ja7mvjFeZqC_se1kcL5nembpyPTI-lwVf7a7dfu3_R5viwE8a0r1p/s1600/IMG_20140709_131440.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi858DMtKrz-OWrOtcSNoy8ThriPTHlJVRzPXugXslRSiAoRRI9V5Ww7iaYXKFh49hqimjh_tohzFNNL1l7oB_gwu-ja7mvjFeZqC_se1kcL5nembpyPTI-lwVf7a7dfu3_R5viwE8a0r1p/s1600/IMG_20140709_131440.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The pH meter, an apparatus which I absolutely dislike using. It's quite slow with detecting the change in pH, so after you've added in your acid/alkali solution it may take like 5 minutes to actually reach it's real pH on the meter. It's definately the part of the buffer preparation which slows me down the most. All the other apparatus, I've gained a technique for being fast and efficient in using them :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhv1I2bLtDlSau0iRjDBoZhyphenhyphenisi5yKP7ghPoW8oxaVwE88FqJKTWUxXv1_M47kNSJG7VP6ym9fBTeKsamMYOMAtxehUZIwmMjU7wVUNS-mNNqot_uiRbDdmhSh79BLvTiXAQgHtUSc3kNzB/s1600/IMG_20140709_131512.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhv1I2bLtDlSau0iRjDBoZhyphenhyphenisi5yKP7ghPoW8oxaVwE88FqJKTWUxXv1_M47kNSJG7VP6ym9fBTeKsamMYOMAtxehUZIwmMjU7wVUNS-mNNqot_uiRbDdmhSh79BLvTiXAQgHtUSc3kNzB/s1600/IMG_20140709_131512.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">All my buffers completed, labeled and dated, proud to see completion :D</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi140RF-JIthglF4rpTnu1WcLXhPT_idsdkEQBd76BzYI3CYxTN4qoNAISGxurKGrAZNJ0QIAvAQnxBfD_thrpm6CMPaHN6KC8MeW4-ygWgZnQdIQvtGt98ZqRTRO4pXrryHwFXiXz_dvWs/s1600/IMG_20140709_131520.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi140RF-JIthglF4rpTnu1WcLXhPT_idsdkEQBd76BzYI3CYxTN4qoNAISGxurKGrAZNJ0QIAvAQnxBfD_thrpm6CMPaHN6KC8MeW4-ygWgZnQdIQvtGt98ZqRTRO4pXrryHwFXiXz_dvWs/s1600/IMG_20140709_131520.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">More labels~</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">MgCl2, used in the buffer prep to aid the further stacking of the thylakoids, to gain a large amount of PSII for analysis.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhqcf3mfBXGTYCW1iQICN-_0R3LHYiJQ79LAqseeaTO4Ja24rpTGDXdrvaiacKccTZitfEwNfgHC-3xyrrk8MMCYXBHx6DUQplh_1TR9KFA8Vi2jUAZaTbFNZUfyl5b3vfWy0S3OaoL47At/s1600/IMG_20140709_131613.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhqcf3mfBXGTYCW1iQICN-_0R3LHYiJQ79LAqseeaTO4Ja24rpTGDXdrvaiacKccTZitfEwNfgHC-3xyrrk8MMCYXBHx6DUQplh_1TR9KFA8Vi2jUAZaTbFNZUfyl5b3vfWy0S3OaoL47At/s1600/IMG_20140709_131613.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">When normal purified water just won't do. There is always PURELAB! An ultra purified water dispenser. Looks very modern and fancy ;D</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">PURELAB homescreen. Literally all you need to do is press the bottom which my index finger is pressing.<br />
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J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-36448618029602230132014-07-05T20:30:00.000-07:002014-08-18T08:20:38.179-07:00Browsing at the science museum<div class="separator" style="clear: both; text-align: center;">
This little blog entry will be about the innovative products which I merrily discovered in the science museum.</div>
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I love the science museum in South Kensington. It's actually the museum connected and really close to my boyfriend's university, Imperial College London. So I'm always in there or end up taking a friend coming down to London for the day :)<br />
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Anyways was waiting for a friend this saturday as we planned to meet for dim sum in South Kensington. She was late so I just wondered into the science museum browsing the shop. There's always cool gimmicks to be found there! Here are the few things I thought were very querky and innovative; products that really caught my attention!<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh8lGw1sV8suAMji6CokfuInqR2jX3jUM_cx85AjWRtxbOzeNsu9mclgfPeANq6aqBlJKQp3fcLQyc3xR_AReAoIHpnOt6HwDbCp9FUE9StJszzZuinB_BbM6mbw-hgY7i214e_JtYfc_oa/s1600/IMG_20140706_120249.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh8lGw1sV8suAMji6CokfuInqR2jX3jUM_cx85AjWRtxbOzeNsu9mclgfPeANq6aqBlJKQp3fcLQyc3xR_AReAoIHpnOt6HwDbCp9FUE9StJszzZuinB_BbM6mbw-hgY7i214e_JtYfc_oa/s1600/IMG_20140706_120249.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">It's a 3 in 1! Chopsticks, fork, knife. This is such a brilliant idea, as many students in the U.K who are constantly saving. Especially with the cost of living as a London student. I'm always trying my best to pack lunch! Something like this would mean I wouldn't have to bring separate metal knifes and fork for when I make salads, sushi, noodles etc. I'm always loving the idea of compact travel items ;)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiqhaFHoliRSIVKMmBM-ORFpCof3GLZxe4CncDQmIp0jkkfO2iJWnLkFu2Pu9p-aEqicSnzF840FEP-Nno3pDzOrdm1qT-FFqPs8ETJ-bFwEH68T03_BUZd049Ole26ir4P0Uq16u9naAam/s1600/IMG_20140706_120256.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiqhaFHoliRSIVKMmBM-ORFpCof3GLZxe4CncDQmIp0jkkfO2iJWnLkFu2Pu9p-aEqicSnzF840FEP-Nno3pDzOrdm1qT-FFqPs8ETJ-bFwEH68T03_BUZd049Ole26ir4P0Uq16u9naAam/s1600/IMG_20140706_120256.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Another innovative packed lunch product. Brighten up any plain drinking water with a zing of citrus. Citrus fruits being rich in vitamin C, benefits such as strengthen bones and antioxidants; helping you to reduce the development of cancers and certain disease. A good source of nutrients such as fibre and calcium! :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEieoeRiVZmivW-YZS1mav12aKMfTKSj6W_ZdFsiK5LTcxf9YswwroImjwNJK2OI2Ebdg3vHeVi12ovo3H45cyxskwqArs45Ec5luQIkAzZidjip5SHzlDNvBRpsWXEmj0czrvoQYMpfAITJ/s1600/IMG_20140706_120329.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEieoeRiVZmivW-YZS1mav12aKMfTKSj6W_ZdFsiK5LTcxf9YswwroImjwNJK2OI2Ebdg3vHeVi12ovo3H45cyxskwqArs45Ec5luQIkAzZidjip5SHzlDNvBRpsWXEmj0czrvoQYMpfAITJ/s1600/IMG_20140706_120329.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Think this lunchbox is designed by the same company as the two products above. I always wanted a lunchbox like this, as at home I often cook alot of salads but salads are quite messy to put in ordinary lunchboxes and once inside such lunchboxes, they no longer look appetizing to eat. However, this lunchbox has a green and white healthy theme going on it which would really complement the rich vibrant colours in salads. Also, the little salad sauce area is great addition, no longer would you need to add your dressing immediately when you make packed lunch. So no wet, awful salads for lunch no more! :3</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhs55kCNwWMDp5BFRb2Kc88LWkPU2wgbt6aVXMAHIJ3q7Mk1clDS9e722qdJF3DPaUFdI5dCbUYjBC6RRTjBVwgWX49_1eIGww7GLmcsp399U1RzERRN8Fh4DN2DSInw8dCbEdzRiJVO_Rz/s1600/IMG_20140706_120352.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhs55kCNwWMDp5BFRb2Kc88LWkPU2wgbt6aVXMAHIJ3q7Mk1clDS9e722qdJF3DPaUFdI5dCbUYjBC6RRTjBVwgWX49_1eIGww7GLmcsp399U1RzERRN8Fh4DN2DSInw8dCbEdzRiJVO_Rz/s1600/IMG_20140706_120352.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Element cups !!!!:3</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgQe7owmrbitS8r5KjfrVM0nxocWXSEouLy1p_YTT2WUXhsvVr8QjXCJYx2moiCH6kSyqc-dnOmZO6Cj7uPCAwfnJSjhUSMOZCqTB5A42KTG4WNBUW1zdAG_g64XyjQRsEaTo_qF6FJgOjB/s1600/IMG_20140706_120401.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgQe7owmrbitS8r5KjfrVM0nxocWXSEouLy1p_YTT2WUXhsvVr8QjXCJYx2moiCH6kSyqc-dnOmZO6Cj7uPCAwfnJSjhUSMOZCqTB5A42KTG4WNBUW1zdAG_g64XyjQRsEaTo_qF6FJgOjB/s1600/IMG_20140706_120401.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Periodic table, think everyone in a biochemistry lab should have one of these. Even if you don't drink hot drinks could be a nice pencil holder? ;D</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiwcXVRmtUR9WoLWCMrjPlmYMUHKCKAk-6UKewdd6mTU-iFH73-2Ma9TAL3JaHt-KaUMDmwxXTrF_FPxtYc0CDLpBSo8f3SDZSff3wyGnB-GnsOEpwB9BX4690J-n4kMhOBnNfdfb30_Aka/s1600/IMG_20140706_120540.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiwcXVRmtUR9WoLWCMrjPlmYMUHKCKAk-6UKewdd6mTU-iFH73-2Ma9TAL3JaHt-KaUMDmwxXTrF_FPxtYc0CDLpBSo8f3SDZSff3wyGnB-GnsOEpwB9BX4690J-n4kMhOBnNfdfb30_Aka/s1600/IMG_20140706_120540.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Salt and pepper shakers.... OMG I LOVE THIS!!! ITS SOOO FUNKY!!! Haha i think anyone who uses conical flasks everyday would find these querky, or maybe find these disgusting in ways? For me I think they're so radical ;D</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5E6zKmraU9-mo79FAsxub80zyH0blJlhB4akoLqYou_nCvB1sRZ-l_ikJZU6T2XLo-LSZf9uR2dkTMLIzxv4qa7p1BaaWzOvD6ZsAHO4xkh_ZH3axx15CSPhBatjj8E-SXrlj5AGjlaAm/s1600/IMG_20140706_120651.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5E6zKmraU9-mo79FAsxub80zyH0blJlhB4akoLqYou_nCvB1sRZ-l_ikJZU6T2XLo-LSZf9uR2dkTMLIzxv4qa7p1BaaWzOvD6ZsAHO4xkh_ZH3axx15CSPhBatjj8E-SXrlj5AGjlaAm/s1600/IMG_20140706_120651.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">So these water stones left on your soiled plants will water your homegrown plants. No need to worry about the watering can again! Looks really cool as well, like a massive contact lense on the soil :O</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiDTh19MZC70yK7jwx_tSRjdnLGigfJwLClCD1u322QeqP71lBE66Q_QM1WHmWUPMmKiB_2254m-VNfabzZ0Vh591LFFzX8f-lk1f2lwVasIntoDmBr-yf8QFHpWckus01Rb9UhTZzs7Z2H/s1600/IMG_20140706_120953.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiDTh19MZC70yK7jwx_tSRjdnLGigfJwLClCD1u322QeqP71lBE66Q_QM1WHmWUPMmKiB_2254m-VNfabzZ0Vh591LFFzX8f-lk1f2lwVasIntoDmBr-yf8QFHpWckus01Rb9UhTZzs7Z2H/s1600/IMG_20140706_120953.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">I'm a big fan of stationary and as you can tell from my labbook blog from before I'm really into colour everywhere. These are just too cute with the pill theme going on. Love it <3</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjO5hQeXOI-DHtAkb6Is_QTvwp8OzZ5KD34a0uKrTp-M0qtYqCW2RjDVhRwWiCq3W85RJSXesfhyy1IvH9SY4OZR-clbenC6Px-KMNaldnIEQSsSzldxVS_OLVhM9I4Tetca4qfvBdP0HdR/s1600/IMG_20140706_121303.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjO5hQeXOI-DHtAkb6Is_QTvwp8OzZ5KD34a0uKrTp-M0qtYqCW2RjDVhRwWiCq3W85RJSXesfhyy1IvH9SY4OZR-clbenC6Px-KMNaldnIEQSsSzldxVS_OLVhM9I4Tetca4qfvBdP0HdR/s1600/IMG_20140706_121303.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Something I probably would buy my child as an aspiring plant scientist :) Even to me this looks very fun as a 20 year old <3</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhTgV5iVaIsW5WoT37k96JDWKHN4BAkOWdy4MFgfKNAQELSvI7DpIBZuFGY60h3g7RY4vs77gbLHBojeMh6t_XfVuhVfVIMcy6LXkYuFJxN0tlQH7ra-8lVBHsAg5Wr7Jb7Qso91I829IZy/s1600/IMG_20140706_121315.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhTgV5iVaIsW5WoT37k96JDWKHN4BAkOWdy4MFgfKNAQELSvI7DpIBZuFGY60h3g7RY4vs77gbLHBojeMh6t_XfVuhVfVIMcy6LXkYuFJxN0tlQH7ra-8lVBHsAg5Wr7Jb7Qso91I829IZy/s1600/IMG_20140706_121315.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This looks sooo funky!!! When I move house I'll probably buy something like this to put on my desk. I just like looking at plants its just ever so refreshing and part of our natural life :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhQvL9gLYhqFpfapbEqxgZeE31L9wpPox6yZzrxMD-vtObV69yjqmarI5KQLpOkYrNMJfSjpZCquWRiNvRWheKAgguVNTQD9aMdrQTu6znW5Plyr3Ns2FDymcyJ1sGMOxJeY4FulSKc62pz/s1600/IMG_20140706_121500.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhQvL9gLYhqFpfapbEqxgZeE31L9wpPox6yZzrxMD-vtObV69yjqmarI5KQLpOkYrNMJfSjpZCquWRiNvRWheKAgguVNTQD9aMdrQTu6znW5Plyr3Ns2FDymcyJ1sGMOxJeY4FulSKc62pz/s1600/IMG_20140706_121500.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Awr, i thought this looked very adorable<3</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiD6BJ7JITti9nsOd9lAoGNUyCVKI-ODjonCOSFOepO4wl6g3q_5AGQ8tbMdMK4mLXZoSWLp-aaxXVY9iOEZ9Z5GI2Jm3TnpCeJrL2-fqp3zsxCmxRDYHRyiDrES4FqrBOpdLUhnaX3-oJM/s1600/IMG_20140706_121717.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiD6BJ7JITti9nsOd9lAoGNUyCVKI-ODjonCOSFOepO4wl6g3q_5AGQ8tbMdMK4mLXZoSWLp-aaxXVY9iOEZ9Z5GI2Jm3TnpCeJrL2-fqp3zsxCmxRDYHRyiDrES4FqrBOpdLUhnaX3-oJM/s1600/IMG_20140706_121717.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">A t shirt with all the % of elements inside the human body. Pretty nerdy to be honest but I would be impressed at anyone who wore such a thing. I would personally make friends :P</td></tr>
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J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-67637532146639931922014-07-03T21:00:00.000-07:002014-08-18T08:20:07.712-07:00Week 6 quick update!<div class="" style="clear: both; text-align: center;">
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<b style="text-decoration: underline;">Quick Blog update:</b> Had meeting with Professor Ruban himself on Thursday and discovered how to further proceed with my project. He wanted me to run HPLC on my B4 fraction from BBY spinach preparation obtained from FPLC. The de epxodiation I yielded in my A9 fractions, containing PSII supercomplex, was sufficiently lower than expected, lower than that of my isolation of stacked thylakoids, which should not be the case. This means something wasn't right with the prep. He told me to check the HPLC on the B4 fractions (Where the Light harvesting II complexes were situated.) De epoxidation in B4 fractions should be 100%. However, with out HPLC analysis we found values much of 70%, therefore we clearly knew something was wrong with the preparation. </div>
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<b><u>Now I know for next time:</u></b> That I must always run HPLC on my B4 fractions, fractions contianing LHCII supercomplexes to see if it is worth the continuation of the BBY spinach preparation past the steps which are identical in protocol to that of isolation of stacked thylakoids.</div>
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<b>Furthermore,</b> the increasing the time of stacking time in the BBY medium. Allow the stacking of thylakoids allows more PSII supercomplexes to be present in the preparation as PSII is mainly found in the stacked thylakoids, compared to that of PSII found mainly in the lamellae of the chloroplast.</div>
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Anyways enough science talk, some nice pictures taken over the week</div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiNDKQIGZQh82vKVCfmIFuAXVOvdNa6H3UK1-HgdqI_tmAbrMWOEUgu7LOgI9an4LiayKLXIPaSWYV_TdEjyW3Y76loTxNaTzrEMXvnoIDtGXSebMIIe74taoe1X7WKFFeqMfnu98CI8XCI/s1600/DSC05032.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiNDKQIGZQh82vKVCfmIFuAXVOvdNa6H3UK1-HgdqI_tmAbrMWOEUgu7LOgI9an4LiayKLXIPaSWYV_TdEjyW3Y76loTxNaTzrEMXvnoIDtGXSebMIIe74taoe1X7WKFFeqMfnu98CI8XCI/s1600/DSC05032.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Irina's healthy growing Peas, wow from 3 weeks they have grown so much! Check earlier blogs to see how they looked at the start! We are just putting wooden sticks in to ensure they grow upright and not tangled :) Happy gardening !</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiCmC6cXQMF7o1CvuqY4hSahVNIZfQT9GpDG7vJGWM4f8EYgqbSk6OamJjBc8XA2txzvDsWfge7yd5dTCmglpkXPqF_CrkmvH_fZHIvDAHnQR8S9YwiIkt0txcHnKFliLmbFC2DLb002EP6/s1600/DSC05034.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiCmC6cXQMF7o1CvuqY4hSahVNIZfQT9GpDG7vJGWM4f8EYgqbSk6OamJjBc8XA2txzvDsWfge7yd5dTCmglpkXPqF_CrkmvH_fZHIvDAHnQR8S9YwiIkt0txcHnKFliLmbFC2DLb002EP6/s1600/DSC05034.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Supervising Irina on HPLC analysis~ teaching her the ways!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjU4PtKSvVV4gDdEEZxa0CS5hJuTOddG3rBC2FD2RQiP-cBe4jR3KIYRF0ruTHZtRg8h-u9Tqn5Chvq5nDzPBJ_qObD7lhnXBzTDo4WFmLKLF2ToPVtOWNw7njvZQa8oBtn71yV8-y1jn46/s1600/DSC05035.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjU4PtKSvVV4gDdEEZxa0CS5hJuTOddG3rBC2FD2RQiP-cBe4jR3KIYRF0ruTHZtRg8h-u9Tqn5Chvq5nDzPBJ_qObD7lhnXBzTDo4WFmLKLF2ToPVtOWNw7njvZQa8oBtn71yV8-y1jn46/s1600/DSC05035.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is how HPLC data analysis usually goes by. We check each peak and compare with the reference spectra for the pigment, which we know due to the position in eluting time in HPLC. This bit is my favourite part of the entire experiment procedure, analysis and interpreting results. So fun! :)<br />
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<b><u>Bit of the side stuff!</u></b></td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5CAHLQ37WuSFpcKPNtGrUSolNKMhYErp2RLbG5oWrcCI_AkHo347Wh7vEsoS4Hd7E0KW-fYigcRyHZqWDWNfUhEHxscpMIwsy4jwwA8_SXPTPrc7nnujf4i1XoI-_UsPMFxP17OarFcDK/s1600/DSC05037.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5CAHLQ37WuSFpcKPNtGrUSolNKMhYErp2RLbG5oWrcCI_AkHo347Wh7vEsoS4Hd7E0KW-fYigcRyHZqWDWNfUhEHxscpMIwsy4jwwA8_SXPTPrc7nnujf4i1XoI-_UsPMFxP17OarFcDK/s1600/DSC05037.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here is Petra grinding up ivy leaves using liquid nitrogen. Yes the steam coming off the ivy is liquid nitrogen that Petra has poured on the leaves, freezing them, making them into crystals, easier to grind into pieces.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgMBm7MmhX4cgn07X00O2gGlSt9U24SUgAxYCh60gUb0xtyYBVFO3N83JJ9fZ8TugetcUfeQp8ETH-8hWu_mKQnyB4LoINAbuWyQbTXEHKR1TfgACrRNuBglV6UlsC1XfyJCvtJVkgBSgmu/s1600/DSC05039.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgMBm7MmhX4cgn07X00O2gGlSt9U24SUgAxYCh60gUb0xtyYBVFO3N83JJ9fZ8TugetcUfeQp8ETH-8hWu_mKQnyB4LoINAbuWyQbTXEHKR1TfgACrRNuBglV6UlsC1XfyJCvtJVkgBSgmu/s1600/DSC05039.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Petra adding the ivy crystals into a test tube ready for it to be grinded by hand. Everything done under iced conditions again.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi82VFB-meJ8CM43qq4LXaivv-z6F3zsTE6Q0jOCjVK3VQwIZ8vvOh29bbYdDonlXqXt7gARNzJf3qpAB2_y7_7d4z4eQ3wr4pkxB9DAgS3DSKuiFBC5j0hXYr9CtOQSru7JtqwZyj7Rj8z/s1600/DSC05041.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi82VFB-meJ8CM43qq4LXaivv-z6F3zsTE6Q0jOCjVK3VQwIZ8vvOh29bbYdDonlXqXt7gARNzJf3qpAB2_y7_7d4z4eQ3wr4pkxB9DAgS3DSKuiFBC5j0hXYr9CtOQSru7JtqwZyj7Rj8z/s1600/DSC05041.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Let the grinding games begin! Apparently Petra hates working with Ivy because they are so hard to grind up and get material from. Did you know Ivy is an incredible plant as they can pretty much grow in any light condition possible. Amazing I love ivy now, like the animal elephant, Ivy is my favourite plant now :) Although i must say spinach is more useful in a sense I can eat them and leafy vegetables are great for the eyes! </td></tr>
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<br />J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-42354862979928741242014-06-30T13:00:00.000-07:002014-07-03T12:42:29.708-07:00Let's talk about lighting, artifical lightingAny form of light is always relevant to this blog, especially because my entire project is about light stress on plants, photosynthesis related. Anyways, this is what today's blog will be about! LIGHT! :)<br />
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It was in the first year of University that I realised the importance of light to the human body. I use to wake up in the winter term feeling all gloomy and depressed, which is quite strange as usually I'm like fully of energy and bursting with happiness. I guess they call it the winter blues ? Anyways, this lead me to discover the condition of SAD, aka. the seasonal defective disorder, which is basically seasonal depression thought to be related to reduce exposure of sunlight. SAD is believed to cause depression by the disruption to the production of important chemicals in the brain's hypothalamus, chemicals of serotonin and melatonin, made from the amino acid Tryptophan. These chemicals are responsible for regulating the sleep/wake cycle, appetite and mood. No wonder the disruption to the normal sleeping cycle, lack of motivation, lack of libido all start to kick in for many of us in in the UK's winter. So it was from such lunch time google session that I discovered the importance of lighting to the human body. I immediately requested a SAD light to be purchased as a gift from my boyfriend. Sadly, I never received the SAD light and spring came eventually~ I moved on with life.<br />
<br />Skip a year and abit on, to my 2014 exam period, I noticed that using different types of lighting in my room really affected my mood/ how long I could actually study for. Traditionally, I have always used an orange yellow emitting light bulb, I never thought much of it till I realised it wasn't my chair that made me feel feel grouchy over a period of time, nor was it the module I was revising. It hit me again that lighting was a big factor in concentration time for me! It sparked back memories to that winter lunch time google session where I found out SAD lighting really helped SAD individuals!<br /><br />So I immediately thought brighter and whiter the light the better for concentration, because that is essentially what SAD lights are made of. Especially, because my room has very high ceiling, for the fact it is an old Victorian house and frantically the room is very large in size. Light does not easily reach the far end of my room where my study is place!<br /><br />So I immediately went out and brought this ecozone bright white light to exchange for the yellow warm tinged study light! I even read reviews before hand how people used this to cure their own SAD in winter.<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhpQ1zAvyW8cxOKRIxsRJWrBgnZkvFxuIYkF2nkORWmXmOdlunfYALYVL45gQhi9ELf-Q6FLFAxGbT8VJJ8ZfDVhIFJATi1rAngo8BZ7UcdB1Vzf60Iz6-tkZ_pJooCaSigsd4NbbFljXbs/s1600/DSC05030.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhpQ1zAvyW8cxOKRIxsRJWrBgnZkvFxuIYkF2nkORWmXmOdlunfYALYVL45gQhi9ELf-Q6FLFAxGbT8VJJ8ZfDVhIFJATi1rAngo8BZ7UcdB1Vzf60Iz6-tkZ_pJooCaSigsd4NbbFljXbs/s1600/DSC05030.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Super bright Ecozone light bulb huh?</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjMUbH5zFt_AVkPe17bVYRtkwTqWeBw8keIcQU3deYOabssm3E11JL8qanZCfX4SsJi6iMJysRH2N9Wpfy3b7JmhndHgSt5yv8vb3iy53v01iCS6rgM2QDOTwHp5_ZmCcF11Vgm-4U9o58l/s1600/DSC05027.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjMUbH5zFt_AVkPe17bVYRtkwTqWeBw8keIcQU3deYOabssm3E11JL8qanZCfX4SsJi6iMJysRH2N9Wpfy3b7JmhndHgSt5yv8vb3iy53v01iCS6rgM2QDOTwHp5_ZmCcF11Vgm-4U9o58l/s1600/DSC05027.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Literally don't even need to switch on the main bedroom light, this was enough. LOOK AT THE GLEAM, how it lights up the entire room~</td></tr>
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So initially it was great, for a week I really felt a difference and was studying for long periods of time. Well longer than with the yellow warm tinge light bulb! I even considered changing the entire house to white light bulbs, the change for me was revolutionary! It made me feel more awake and energised, essentially it felt like being outside, but indoors. Text on my lecture printouts were very crisps as well thank to the lighting, everything was great.<br /><br /><b><span style="font-size: large;">BUT.... </span></b>there is always a but to everything. Nothing is a happy ending like fairy tales I guess.<br />
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I slowly felt a bit sick with the white light intensity. Yes it could of been due to the fact I was constantly studying all the time and revision eventually makes you feel a bit sick, but I took breaks and coming back I still felt sick. It wasn't like a mental I feel sick of revision because I revised too much today. I generally have quite strong will and I knew I had to study hard for good grades, so I would always be consciously aware about such thought. No, I felt physically sick, that was when I knew artificial white light must be bad for you. SO there I went, with my usual, exquisite mind, eager to find out proof for any theories I connect together....Is white light bad for you?<br /><br />Reading from site to site, paper to paper. I came to the conclude it actually was...<br />
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Turns out exposure to white light, suppresses your melatonin production by x5 than that to the normal yellow warm tinge light bulbs! This is due to the fact that white light bulbs emit a shorter wavelength than that of ordinary warm yellow tinge bulbs! Gosh I was shocked! Apparently, the U.S government are aware of this and make sure that excessive amounts of light pollution are not introduced in public areas.<br /><br />Melatonin as expressed above is very important for the body's biological clock. In addition, it has anti cancerous and anti oxidant properties too. I immediately changed back to my yellow warm tinged light bulb and to be honest I didn't feel physical sick anymore. Yes I couldn't study as long, but sometimes it is better to take more breaks really. It's actually been proven that interval learning will aid your memory more likely than that of mass training. Something I learnt in my neuroscience module this year.<br /><br />Here is the link to the paper itself, explaining why interval learning is better than mass, just in case you are intrigued :)<br /><br /><u style="font-weight: bold;">Click here:</u> <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1934342/">A functional MRI study on associative memory</a><br />
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<br />J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-2932334809932441222014-06-28T18:00:00.000-07:002014-07-01T13:12:15.448-07:00Back to Sheffield!<div class="separator" style="clear: both; text-align: center;">
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Home visit, yet again! Always need to go back and see the folks! In fact this time it was my brother's birthday so I went back for that reason. Anyways, so some photosynthetic related products while shopping at Meadowhall (It's sheffield massive shopping centre, really good place to go shopping, everything under one rooft! Totally recommend it :)) I'm not sure if it is the fact I'm sciencey that I see these project related products when I go out. Or maybe it's the law of attraction, because I'm always thinking about my studentship in the back of my head, so it just brings me closer to these things? Signs from god? :P</div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgE8W1CW9emBKOM79esPL-logTdHOdiXr45E9e9ZQtqTA-vIMQFD979ZTgo-QBRDCCE-d8cXKcIk_bfgcEAAt7c3tJtssGwV5mxNtPTmlfzQ_6v1VypL2JAC1PsdG5euclftgeoFdFXOSpx/s1600/IMG_20140628_193719.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgE8W1CW9emBKOM79esPL-logTdHOdiXr45E9e9ZQtqTA-vIMQFD979ZTgo-QBRDCCE-d8cXKcIk_bfgcEAAt7c3tJtssGwV5mxNtPTmlfzQ_6v1VypL2JAC1PsdG5euclftgeoFdFXOSpx/s1600/IMG_20140628_193719.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Oh look these could almost pass as spinach leaves, but in rubber form! So cute :3 Quite refreshing to see such a rubber to be honest. Yeah this stationary shop sold all types of rubbers! </td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhW2Ou-smwbvR8NGZTy3WGhRCAKpNNS-CJFsZ-CI0a8d5LtrFKEbHMYjBG_Cku9ddRSsdnjHgE3LPLtvq8_U8HFr585DnzW3L8pewYjghRlMnvPAMcrwi2mN5FefEJJD_qtaffVnbCMKoo6/s1600/IMG_20140628_194200+-+Copy.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhW2Ou-smwbvR8NGZTy3WGhRCAKpNNS-CJFsZ-CI0a8d5LtrFKEbHMYjBG_Cku9ddRSsdnjHgE3LPLtvq8_U8HFr585DnzW3L8pewYjghRlMnvPAMcrwi2mN5FefEJJD_qtaffVnbCMKoo6/s1600/IMG_20140628_194200+-+Copy.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption">This is a product I observed in Red5 the shop. Love this shop! It is not just for guys, its for girls too, especially girls like me! I'm really into future tech stuff, especially if it aids in sustainable living or makes your life easier ;D<br />
<b><u><br /></u></b><span style="font-size: small; text-align: start;"><b><u> Here is a product description & link</u></b></span><b><u><br /></u></b><br />
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<b><u><a href="http://www.red5.co.uk/6-in-1-solar-kit.aspx">6 IN 1 SOLAR KIT</a></u></b></h2>
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<span style="background-color: white; color: #666666; font-family: Arial, Helvetica, sans-serif; font-size: x-small; line-height: 16.00299835205078px; text-align: justify;">The Sun. A massive ball of fire burning hydrogen in to helium about 93,000,000 miles away. It roughly takes 8 minutes for light to travel here and it seems a shame for it to come all that way and not get used to its full potential! That's why we were really excited about the 6 in 1 Solar Robot.</span></div>
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: x-small;">This 'do it yourself kit' is a great beginners introduction to solar power technology and the end result is your very own little robot. There are 6 different ways to build it, so you could have a robot dog one minute, or a boat for your bath the next! If either of those don't do anything for you then why not build your own little plane (moving or still), windmill or solar powered car!</span></div>
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: x-small;">The good news is that you don't need a degree in robotics to start with, or have to try and figure out which screw is A678b and how part D456 slots into F9097a!! The diagrams are big and very easy to follow, there are only 37 parts and no screws are involved!</span></div>
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: x-small;">Sounds great so far, but what if you live in the UK, like us, which hardly ever see's the sun? Well, this eco friendly robot can also be powered by a 50 halogen bulb (or brighter).</span></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjJCAG25UmIkLNgFrIUAwISxQzjhZMeeAWFhkvvpmEYmVmKvQ64NImM6J_czkR1CtOqmNpRrsXx1ehkE3f7agKfwzKEvrH82NiGK30rzscwd2DqJp1jrw444ISp4ZMFbLzyuJjV38TrnGpK/s1600/IMG_20140628_194212+-+Copy.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjJCAG25UmIkLNgFrIUAwISxQzjhZMeeAWFhkvvpmEYmVmKvQ64NImM6J_czkR1CtOqmNpRrsXx1ehkE3f7agKfwzKEvrH82NiGK30rzscwd2DqJp1jrw444ISp4ZMFbLzyuJjV38TrnGpK/s1600/IMG_20140628_194212+-+Copy.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Another cute solar product! Maybe one day these products will be developed so people can actually purchase them to generate massive amounts of energy via the sunlight (probably never in the UK as we don't get enough sun, but places like Egypt & India! ^^)</td></tr>
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<b><u> Here is a product description & link</u></b><br />
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<b><u><a href="http://www.red5.co.uk/solar-recycler-6-in-1-kit.aspx">SOLAR RECYCLER 6 IN 1 KIT</a></u></b><br />
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<span style="font-family: Arial, Helvetica, sans-serif;">It’s time to peel your kids (and own) faces from the TV screens and get them playing back in the real world. This cool building set not only increases creativity and brain development but also has a high impact on fun levels and planet saving power.</span></div>
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<span style="font-family: Arial, Helvetica, sans-serif;">All you need to build an exciting new toy is this kit, a fair bit of sunlight and a few pieces of rubbish. Not only are you helping the planet by using its natural solar power but you can recycle old cans, CD’s and plastic bottles. Not a single battery is needed to build the six fun models, just eco-friendly solar power. Inside your kit is a set of easy to follow instructions and diagrams explaining clearly how to install and utilize recycled materials. Turn your old CD’s into a speedy race car, make a used bottle into a flying bird or convert an empty can into the ultimate drumming robot.</span></div>
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<span style="font-family: Arial, Helvetica, sans-serif;">The Solar Recycler 6 in 1 Kit is a fantastic way to enjoy learning about the science of renewable energy and gives the imagination a huge boost.</span><br />
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So going to buy my children these when I get around having children somewhere in the far future. Or anything that is remotely similar in the future :) Why give your children Barbie or Cars? When you can give them science experimental kits and Lego for creativity development in the mind ;) </div>
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J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-28900702040069129072014-06-19T12:33:00.000-07:002014-07-01T12:46:36.427-07:00HPLC 2nd run!<div class="separator" style="clear: both; text-align: center;">
So today we did HPLC runs on our BBY spinach collected fractions from FPLC performed yesterday, to analyse the pigments in PSII supercomplexes.</div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhIJEtl92cQL-SkEbGHiBlk9nGBi9Yrmju2lTDidCjOSadQGE1mGL68P2GulaDjAsMHnYkt-wWzXUe_iMXZllQZIw80Ll4WFpEgjVDYiHSIZtJC2XCk7KrhBWS10JCclomp6_okWaM9KO1J/s1600/DSC04904.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhIJEtl92cQL-SkEbGHiBlk9nGBi9Yrmju2lTDidCjOSadQGE1mGL68P2GulaDjAsMHnYkt-wWzXUe_iMXZllQZIw80Ll4WFpEgjVDYiHSIZtJC2XCk7KrhBWS10JCclomp6_okWaM9KO1J/s1600/DSC04904.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is a microconcentrator which we place our medium in to aid concentrating it, by using a centrifuge. </td></tr>
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<tr><td class="tr-caption" style="text-align: center;">HPLC machine again :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgmJCj4JsBexKhiCtZiaZKUEMFXm6It3L5Y05_yGzACZXZqZeccx-eImb4d15Y9FqQ4YpQMLfD5ifHYtwsCtISmL66iFsmrcYPT3-hVQwfNM2EaL109d_Y-BKCUrHHTcfH5t3GkJxuX_ARF/s1600/DSC04907.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgmJCj4JsBexKhiCtZiaZKUEMFXm6It3L5Y05_yGzACZXZqZeccx-eImb4d15Y9FqQ4YpQMLfD5ifHYtwsCtISmL66iFsmrcYPT3-hVQwfNM2EaL109d_Y-BKCUrHHTcfH5t3GkJxuX_ARF/s1600/DSC04907.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">My favourite part is adding the labels to the vials for HPLC, its so intricate task, love it!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi06uxZJbpY-k4EGJmfyBKWIykF-BpPPSBtKgQu7w3Xo57FiwkEbfhyphenhyphenBzL9YVCfJ5r6jxXKOeWzdqQNIeAN4B2dtPzdsifyMvgrwS0q0YqVDTZo0viZx4xd2HJ2nGvroiDu8RHTC7WrIhgv/s1600/DSC04909.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi06uxZJbpY-k4EGJmfyBKWIykF-BpPPSBtKgQu7w3Xo57FiwkEbfhyphenhyphenBzL9YVCfJ5r6jxXKOeWzdqQNIeAN4B2dtPzdsifyMvgrwS0q0YqVDTZo0viZx4xd2HJ2nGvroiDu8RHTC7WrIhgv/s1600/DSC04909.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Pipetting our medium into the microconcentrator!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhk1Smhhr5PPRxorSx6hpkb4igSHNOsFpm7Tc98bwgEvq6cz_GIlye74Tzd5kZMdjSa6GBwxMzrxMFW9E-0SzrE9OT8v-U6xt3qpge1NW5Yh4EfduC2TTI1D6wUv2IivD66BNH_g-5md5eX/s1600/DSC04910.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhk1Smhhr5PPRxorSx6hpkb4igSHNOsFpm7Tc98bwgEvq6cz_GIlye74Tzd5kZMdjSa6GBwxMzrxMFW9E-0SzrE9OT8v-U6xt3qpge1NW5Yh4EfduC2TTI1D6wUv2IivD66BNH_g-5md5eX/s1600/DSC04910.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The white lids placed on the top of the ependorf tubes are filters, as discussed before we use syrines to inject our sample into the ependorf tube, this technique allows us to filter our medium! </td></tr>
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Went home and stuck in my results from HPLC from today's session. I wanted to edit the files to nicely fit in my lab book and the fact that the windows 2000 interface is just SO slow on the lab computer, makes more sense to do it at home.<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiO5o7wvJIU_asiMvTYKZUCnK-_iwhCPsrvOkVIiFFezaZsRf9imtD0jSnUPdBZYfoaugoM2fjfPOeDp5ED572Z66aqS4af8emt1oApMEu-_RNukFW3q_xrjx2VTm7vmRniYUFl2qce3QuR/s1600/DSC05024.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiO5o7wvJIU_asiMvTYKZUCnK-_iwhCPsrvOkVIiFFezaZsRf9imtD0jSnUPdBZYfoaugoM2fjfPOeDp5ED572Z66aqS4af8emt1oApMEu-_RNukFW3q_xrjx2VTm7vmRniYUFl2qce3QuR/s1600/DSC05024.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Love my Pritt stick, its what makes my lab book so informative, sticking in all the files!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhV1rQW3501U8tgxaBATFNAw5VnxAd2WNWuQInKNEDf0vE9c-SIRMoZZUpqMDxRff1_w6XlfszyQKuiFbglapanCBHANeUZjIcS-ZQc58qKVzWy7tS9mjeG-VbLcmpNADL8doR0xtM5kJGa/s1600/DSC05025.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhV1rQW3501U8tgxaBATFNAw5VnxAd2WNWuQInKNEDf0vE9c-SIRMoZZUpqMDxRff1_w6XlfszyQKuiFbglapanCBHANeUZjIcS-ZQc58qKVzWy7tS9mjeG-VbLcmpNADL8doR0xtM5kJGa/s1600/DSC05025.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Results from #2 run of HPLC :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgMpAPb0ptt9mQbMxD-wgkujkVLGdFsrINnqMJxttRZ7k-7ILVBHHZToC0UCjrAF6wwNYHq5PxQdlQuyrTIT5o7snSD_c7YWj02MJx0oOjsmSB3lGk5nREU_SKjWARrJZAMF4Am1KzWkmZR/s1600/DSC05026.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgMpAPb0ptt9mQbMxD-wgkujkVLGdFsrINnqMJxttRZ7k-7ILVBHHZToC0UCjrAF6wwNYHq5PxQdlQuyrTIT5o7snSD_c7YWj02MJx0oOjsmSB3lGk5nREU_SKjWARrJZAMF4Am1KzWkmZR/s1600/DSC05026.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">I absolutely love my beloved red canon printer. Scans, copies, prints, duplex. Duplex is a big for me for lecture notes and it's ecofriendly to, save paper! I absolutely adore my printer to bits, haha !<br /></td></tr>
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<br />J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-53254402565831994822014-06-18T22:00:00.000-07:002014-07-01T12:14:51.375-07:00A Super Long Day: BBY Spinach Protocol<div class="separator" style="clear: both; text-align: center;">
<b>So today was an extremely long day in the lab. I was in from 8am-6.30pm...</b></div>
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The length of the BBY spinach protocol is why we started off with the isolation of stacked thylakoid preparation first, to see if it yielded high amounts of PSII super complexes. However, no matter what, the PSII super complexes were not pure in the sense that the PSI was still always attached onto them, which is not what we wanted. We only wanted PSII super complexes to analyse the pigments in the Xanthophyll cycle under light stress. Therefore, we took the the protocol of BBY spinach, which involves more steps, but the isolation of ONLY PSII super complexes is the final outcome- an outcome which we desire.</div>
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Due to the fact today was a long day, I took many photos to keep me going & the 2 shots of coffee I took at 7am! (Warning you, there are many photos to follow, to the end of the page!)<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiTpQiR03eRbw38BBlTA4jqd7yg8-KPTcRcFp1YjmpszkyJTDJePVcoNgZOgtU8zn2b5Pln_82qO7X47wSIw1Bp3n5d-8x1eLdnr8-78sDaG9fMzjh_KnX7JjNUYcVNR5lwmWtT-jOr1o2R/s1600/DSC04823.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiTpQiR03eRbw38BBlTA4jqd7yg8-KPTcRcFp1YjmpszkyJTDJePVcoNgZOgtU8zn2b5Pln_82qO7X47wSIw1Bp3n5d-8x1eLdnr8-78sDaG9fMzjh_KnX7JjNUYcVNR5lwmWtT-jOr1o2R/s1600/DSC04823.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Preparing the muslin filter ate for the grinded slushy spinach leaves via the polytron. The protocol of BBY spinach starts off identical to isolation of stacked thylakoids.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgB0-9Rekjx3fylt4CztdIOMi1pZKwC9OKw1tnQlbQ19ZG-Q9avql3xRjNjz7Ymxyz2thDpDNy-pQ4IQqg7wKZCDOPv4yLDzt-APNqMhRaiwR1E8ue5oUXLOPbWEp6s6BoK0kjAJEfsOUIu/s1600/DSC04824.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgB0-9Rekjx3fylt4CztdIOMi1pZKwC9OKw1tnQlbQ19ZG-Q9avql3xRjNjz7Ymxyz2thDpDNy-pQ4IQqg7wKZCDOPv4yLDzt-APNqMhRaiwR1E8ue5oUXLOPbWEp6s6BoK0kjAJEfsOUIu/s1600/DSC04824.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Irina & Petra walking down the fourth floor corridor. Irina is a 2nd year biochemistry student from Queen Mary. She is doing her third year project with Ruban and basically is shadowing/ learning techniques before the start of her project! So glad she is here, more people in the lab more fun, as she is quite social so there's always something to talk about. Labs are always so fun in our team: Me, Irina, Petra and Erica. We have good chemistry, chemistry is so important. Can't imagine how my studentship would be like if it wasn't like this....</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEggWe8DODf6qq2962Wspk8P3YXbiMST24p2RM3ZbQvl5Aek1oHsFF7a0GmsZFeeycJ3OUeKlpHQJrD-T3IkbmJa6s5uhykNpc4NOPLmraIdAnrDuf8DI0Feoe-JdD58p1DnXtVGV2q7KQPM/s1600/DSC04825.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEggWe8DODf6qq2962Wspk8P3YXbiMST24p2RM3ZbQvl5Aek1oHsFF7a0GmsZFeeycJ3OUeKlpHQJrD-T3IkbmJa6s5uhykNpc4NOPLmraIdAnrDuf8DI0Feoe-JdD58p1DnXtVGV2q7KQPM/s1600/DSC04825.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Fourth floor corridor, offices and labs ~</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicJi9808YXGhTWrPjXzXt1pMzDClLGeI9iqf63ZEKkFq-g4IkR2K6RhdnPWMlq8bnnxtGOYmSOwnSWmG134GjTawEBTxSJ0CqznAgzTwo8l4FOfjkuO8TwcaIduvj6OSyjHH4VzJySrdvt/s1600/DSC04826.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicJi9808YXGhTWrPjXzXt1pMzDClLGeI9iqf63ZEKkFq-g4IkR2K6RhdnPWMlq8bnnxtGOYmSOwnSWmG134GjTawEBTxSJ0CqznAgzTwo8l4FOfjkuO8TwcaIduvj6OSyjHH4VzJySrdvt/s1600/DSC04826.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Walking up the stairs~</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh0pFNSHVVl8k1md7fgKl-u6TVQTJZjNKxEBui2q5gPB-fUUfeWrA9ITfTnW8jAy_meddx84NPT5bld3JK-4NBOAbo0pvs29LOxuZiqgfSpt0nlUAlNHjQY8P-YbvUZaUf9WMMtRj59v4ZG/s1600/DSC04827.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh0pFNSHVVl8k1md7fgKl-u6TVQTJZjNKxEBui2q5gPB-fUUfeWrA9ITfTnW8jAy_meddx84NPT5bld3JK-4NBOAbo0pvs29LOxuZiqgfSpt0nlUAlNHjQY8P-YbvUZaUf9WMMtRj59v4ZG/s1600/DSC04827.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Irina university grown peas! Her 3rd year project will be involving peas so she has to grow them! It's quite nice how she can grow hers and see her material from start to end. Due to the fact that my material is spinach and we buy from Whitechapel market, I have no control over the conditions the spinach are grown in! Obviously, every factor matters in science and we like to control as much as we can :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgudSG_mWMdKfqXZeRFOsPtxWHCKVFeZNbIJJHaAQy87IbkIZPLNnM1zzswjHYruLUUMkUaslPtsl9ZfPm7ArH5qgaoUMxuG4cx72KczqLmbezFqVkYQZIxSuVH3OYnfPInvI1SlUIMXnxb/s1600/DSC04828.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgudSG_mWMdKfqXZeRFOsPtxWHCKVFeZNbIJJHaAQy87IbkIZPLNnM1zzswjHYruLUUMkUaslPtsl9ZfPm7ArH5qgaoUMxuG4cx72KczqLmbezFqVkYQZIxSuVH3OYnfPInvI1SlUIMXnxb/s1600/DSC04828.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Such a fine day on Fogg's rooftop ! Love the colour of the sky.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjReyQc4iPYDQra0yXkI6Z1Fv5zKHujxwliEzfPfRnbOIh1gE55uVsiOjjlxMVnEYp0CsXzMLSh4QR0ETNO2pR9drPwWE_GO52OBkSCtvucPw8qGEdjP5-Fk3uhK0KKz9pE8VhWX8afxXyn/s1600/DSC04829.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjReyQc4iPYDQra0yXkI6Z1Fv5zKHujxwliEzfPfRnbOIh1gE55uVsiOjjlxMVnEYp0CsXzMLSh4QR0ETNO2pR9drPwWE_GO52OBkSCtvucPw8qGEdjP5-Fk3uhK0KKz9pE8VhWX8afxXyn/s1600/DSC04829.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">A fig tree grown by Anna, phd student at Ruban's Lab. Quite impressive right? Looks so healthy ;)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiv0XLCW1ZoVdujSkVSqRddxaBaDAVP_Sglf7BxUuCPM0W1ftWDsxl0fcXz1DbZwphI3a5ur9z4BrH23AP7B-wlCfy5Z00ZArTlTYhdfE94aIkuhHgHYw2hyphenhyphen_z305WOEPtSDlCS_OUjJfSR/s1600/DSC04830.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiv0XLCW1ZoVdujSkVSqRddxaBaDAVP_Sglf7BxUuCPM0W1ftWDsxl0fcXz1DbZwphI3a5ur9z4BrH23AP7B-wlCfy5Z00ZArTlTYhdfE94aIkuhHgHYw2hyphenhyphen_z305WOEPtSDlCS_OUjJfSR/s1600/DSC04830.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Adding the spinach leaves from the freezing cold icy water into the glass square cylindrical column, getting it ready for grinding with the polytron!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYV7RiEqU_1pjgdpWm8hODuIuPUikdesHqPoCQo6ukgN3nOehQ35nM8uHXfrgAoWX07akzYPqypq50TSIw7gdfAz11FRjaYSv-axF7XlG8yJyTJ3n-Zrbn17cBx-t8s0MJRKYSRFXEJfmA/s1600/DSC04831.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYV7RiEqU_1pjgdpWm8hODuIuPUikdesHqPoCQo6ukgN3nOehQ35nM8uHXfrgAoWX07akzYPqypq50TSIw7gdfAz11FRjaYSv-axF7XlG8yJyTJ3n-Zrbn17cBx-t8s0MJRKYSRFXEJfmA/s1600/DSC04831.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">OH GOSH THE GREEN IS JUST SO LUSH! Love this photo, its ever so vivid ;) So yes filtering.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh74MInlb6hcpNqOg2zGq1enOxbDdis4pu3Ugo-b-6PS57syLWXspIeQwd-ad5zFP1CuOfkYy9L6xWFxnrf4Q8YTcoVkj5bmg9cQUOK7Gs6M8Hf5dKQZES07AG7YJGT0Akq7hfFs9G63mdu/s1600/DSC04832.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh74MInlb6hcpNqOg2zGq1enOxbDdis4pu3Ugo-b-6PS57syLWXspIeQwd-ad5zFP1CuOfkYy9L6xWFxnrf4Q8YTcoVkj5bmg9cQUOK7Gs6M8Hf5dKQZES07AG7YJGT0Akq7hfFs9G63mdu/s1600/DSC04832.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Squeezing the filtrate to get all the liquid out, you can only do this in the first filtrate as the second time filtering, you just let it filter drip.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjj8oglMINsRW5p5FujpX7sX6Rx06DScwmqPr8NNz73GjH5q-fXF7-AMOb41llle8QH2Os2U65AV02FpEQkDxVJncbbbmGDTRn5wRpV06KnF1yRD5ZPw6nLeiQHHryWwjYf8JojOBR5p04T/s1600/DSC04834.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjj8oglMINsRW5p5FujpX7sX6Rx06DScwmqPr8NNz73GjH5q-fXF7-AMOb41llle8QH2Os2U65AV02FpEQkDxVJncbbbmGDTRn5wRpV06KnF1yRD5ZPw6nLeiQHHryWwjYf8JojOBR5p04T/s1600/DSC04834.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">#2 filtering </td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhaAl6vOdikkhs6AcmvoJCSGOsAbzbq0t9TnVOXayMrGz7np0YlH5ysy9p2mGCFbB9O4-Lq-0YrKCz3_nTKjg-FUtQO_23RYBTaNwmqtSp4SmJkAGmZUPf2RKpbP1ZAeINzz7qVsymJOIne/s1600/DSC04835.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhaAl6vOdikkhs6AcmvoJCSGOsAbzbq0t9TnVOXayMrGz7np0YlH5ysy9p2mGCFbB9O4-Lq-0YrKCz3_nTKjg-FUtQO_23RYBTaNwmqtSp4SmJkAGmZUPf2RKpbP1ZAeINzz7qVsymJOIne/s1600/DSC04835.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Helping each other with the filtrate.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgi26B3nWLUlT1umGqegC0RuRCJzd7gh4_rau62TVMniFln6BPh8TxgitYgJd7d26zq0GHmXB4F8-BbQSQYnNobMRKxouKZ374_e70TTQc1gvNfY04siEvPYz9WsSy2H9Et7uRygNWxIuNo/s1600/DSC04836.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgi26B3nWLUlT1umGqegC0RuRCJzd7gh4_rau62TVMniFln6BPh8TxgitYgJd7d26zq0GHmXB4F8-BbQSQYnNobMRKxouKZ374_e70TTQc1gvNfY04siEvPYz9WsSy2H9Et7uRygNWxIuNo/s1600/DSC04836.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Adding solution in a filtering system. Later you will see how we filter the solution with pumping air outwards using a pump attached onto the de gaser equipment. Which rapidly forces the liquid to filter through the pores.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjA_CzW6wtfl-0cisvsCvIrHEp4IeXEX0CoYWFKd-7oupFHooDfZb8ss36d2NePionYpgzehRnHgbowbBeEvavVGc_5UjXnEcC8N-BWVStRHLuci5-GLuvaq4fT9M6B3poih8GMLbEJER0E/s1600/DSC04839.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjA_CzW6wtfl-0cisvsCvIrHEp4IeXEX0CoYWFKd-7oupFHooDfZb8ss36d2NePionYpgzehRnHgbowbBeEvavVGc_5UjXnEcC8N-BWVStRHLuci5-GLuvaq4fT9M6B3poih8GMLbEJER0E/s1600/DSC04839.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Measuring the O/D. Allowing us to calculate the chorophyll A: B ratio with such measurement. </td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjXygGd-oUy8PlJdqX23lU3sCzmXpyDmSAW3vYb1XtoW_e5Hty0a0wvUIeahz4xCYaleC4U-hGCe1ieVhJkYZJ0oCFHx6bB3_mgaVDSzpWaxdh3jm2XTeHmUjrw7G4aPkqg6nabJaO8m0w_/s1600/DSC04841.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjXygGd-oUy8PlJdqX23lU3sCzmXpyDmSAW3vYb1XtoW_e5Hty0a0wvUIeahz4xCYaleC4U-hGCe1ieVhJkYZJ0oCFHx6bB3_mgaVDSzpWaxdh3jm2XTeHmUjrw7G4aPkqg6nabJaO8m0w_/s1600/DSC04841.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The blue slot is for the reference, acetone, what we added to our solutions extra.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgFtx-wV5DwGDYJOsCK0SOh_9K4tDr7QssdzM2Fv7poJ6atpgOewdU0YCN7vgiPGLqnTqv1F9NCw8MtbhswWkkCxO-khojFZh1Ax2E_bVV8tpD316leAjxorkuF89v-ZNac3MAH9vApcgIF/s1600/DSC04842.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgFtx-wV5DwGDYJOsCK0SOh_9K4tDr7QssdzM2Fv7poJ6atpgOewdU0YCN7vgiPGLqnTqv1F9NCw8MtbhswWkkCxO-khojFZh1Ax2E_bVV8tpD316leAjxorkuF89v-ZNac3MAH9vApcgIF/s1600/DSC04842.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Keypad for the spectrometer.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Pouring our solutions into the glass columns after centrifugation.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiruHyYajGgSLxI1NEuBQ6TqV9iq5q8gDzJ1XM2NLc4JiK8F9E7_yoIAk_k5xcLhJQXgwMXzp3IT1TtBoHePdjJ8170MwNA24-cEebFUO7HL_cHsbe9o66zTOf7HboosEhR5mserTIYK4fJ/s1600/DSC04847.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiruHyYajGgSLxI1NEuBQ6TqV9iq5q8gDzJ1XM2NLc4JiK8F9E7_yoIAk_k5xcLhJQXgwMXzp3IT1TtBoHePdjJ8170MwNA24-cEebFUO7HL_cHsbe9o66zTOf7HboosEhR5mserTIYK4fJ/s1600/DSC04847.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Nicely drawn table for recording the O/D of my solutions.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj5woeKKOTrKjCDNvZ3jqFRcOPraMaagSlCenRDVd04OsAfIXrBeRpgBt8HQDcjJJb3KqBE3_dAMbYoTwvbmYhAkQkQeLdnMrZQw4zFtib7f8Ylxf0KO2UTVoXgiSw_trzbdycE8zueZbGe/s1600/DSC04849.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj5woeKKOTrKjCDNvZ3jqFRcOPraMaagSlCenRDVd04OsAfIXrBeRpgBt8HQDcjJJb3KqBE3_dAMbYoTwvbmYhAkQkQeLdnMrZQw4zFtib7f8Ylxf0KO2UTVoXgiSw_trzbdycE8zueZbGe/s1600/DSC04849.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Moved labs where we shall be de gasing the solution so it will filter through rapidly. Petra is sorting out the pipes for vaccum out the air.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgrVH4XIWflt9_LWxpGlat2eVonJ4dVLSEp-GfiDAnfpIOma3pVbFPLoQv5Fg6N5AV7R7IoOpJfD2z8cwUPm13z4F8LoWEMlNr6ImM-azv9EcIfWnfza_Rz-vb9goXNqtFSAMxqBTzmwil-/s1600/DSC04851.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgrVH4XIWflt9_LWxpGlat2eVonJ4dVLSEp-GfiDAnfpIOma3pVbFPLoQv5Fg6N5AV7R7IoOpJfD2z8cwUPm13z4F8LoWEMlNr6ImM-azv9EcIfWnfza_Rz-vb9goXNqtFSAMxqBTzmwil-/s1600/DSC04851.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Me holding onto the pipe which vaccums out the air. As you can tell already the liquid on the top has rapidly filtered all the way through.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhsmc2TJENupBgoNYQvHEdhoMvehfG_sFzxvWKcXj1b18X1ICDodt8NQInn1Odbzi6kc6P1T3Y5UVZf2QjuAg5kBWbmuk7WImIUCs7CMC9Z09wk4Omyg8IkenZ1WxkOV-DghhC-0f8YsywH/s1600/DSC04855.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhsmc2TJENupBgoNYQvHEdhoMvehfG_sFzxvWKcXj1b18X1ICDodt8NQInn1Odbzi6kc6P1T3Y5UVZf2QjuAg5kBWbmuk7WImIUCs7CMC9Z09wk4Omyg8IkenZ1WxkOV-DghhC-0f8YsywH/s1600/DSC04855.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">We have to do this knee tapping technique to get rid of the bubbles at the bottom of the flask. Alot of bubbles are attached to the magnetic stirrer which is required when we are making the buffer solution, adding all the powders.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgTgrB5ZdonYofps42_74cbDpAfxWI8jMS6VMs6hehmt5kYg3iPmcHutFLAZb3GfbbuolZQuKDPHcb9bDQS1mKacdECMqOpwmXhdvHivbI57w4CDOxwdM4gf-hpoLYfuvI1ftydZQPdDxNk/s1600/DSC04857.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgTgrB5ZdonYofps42_74cbDpAfxWI8jMS6VMs6hehmt5kYg3iPmcHutFLAZb3GfbbuolZQuKDPHcb9bDQS1mKacdECMqOpwmXhdvHivbI57w4CDOxwdM4gf-hpoLYfuvI1ftydZQPdDxNk/s1600/DSC04857.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here is Ruban's dark lab! Instrumental !</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgYwM1F3f4NmNJrn1GnVEBh0WxU_ETDBwYnuqf8eod4ZyohmCLHHEtwFBZDqpvUBlkgZyo9i6UmmxoqB3xkL5x5cP55RU8NovHQpkuiRe6hryT6PJqxEEC_m1R-a2eUYY3W7LC3OJU0GV39/s1600/DSC04858.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgYwM1F3f4NmNJrn1GnVEBh0WxU_ETDBwYnuqf8eod4ZyohmCLHHEtwFBZDqpvUBlkgZyo9i6UmmxoqB3xkL5x5cP55RU8NovHQpkuiRe6hryT6PJqxEEC_m1R-a2eUYY3W7LC3OJU0GV39/s1600/DSC04858.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here is where all Ruban's researchers are placed. Erica, Petra, all the phd students: Anna & Max.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjivhCiVP_5b6pcKNwWf8YpcDB7ZlQIezZlGZjqX227NCvbhPJJAf7BSpO-NrgAxDzg1La3mcBlZt1IVWjHNkZpds8c5vVltsl7j12rzcVf_e8IvK074niebEyooOWtCONnD3HIRR5jmIgq/s1600/DSC04859.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjivhCiVP_5b6pcKNwWf8YpcDB7ZlQIezZlGZjqX227NCvbhPJJAf7BSpO-NrgAxDzg1La3mcBlZt1IVWjHNkZpds8c5vVltsl7j12rzcVf_e8IvK074niebEyooOWtCONnD3HIRR5jmIgq/s1600/DSC04859.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Biochemistry lab~ one which i've talked about already. I so prefer being in the biochemical lab there usually some form of light coming through compared to the dark lab. The dark lab just makes me feel sleepy during the day, as its pretty much blackout in there!<br />
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<b><u> Lunch time has arrived! So here is how my lunch is usually spent!</u></b></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjeKF-10EGMTbIGubxBZUd15azn7pvVR91WihRKwRLreTAhn7jIkw9Qjbrw9Boxs6iuxrzbFlXOvhQ048p9xaN7Kj8Nxn3ub0nkGfT6sWWVha4rcEmG8v3aj7LAok60d7P_pBVrF8L9WZRG/s1600/DSC04860.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjeKF-10EGMTbIGubxBZUd15azn7pvVR91WihRKwRLreTAhn7jIkw9Qjbrw9Boxs6iuxrzbFlXOvhQ048p9xaN7Kj8Nxn3ub0nkGfT6sWWVha4rcEmG8v3aj7LAok60d7P_pBVrF8L9WZRG/s1600/DSC04860.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Finally I have manage to take a picture of the Fogg Building, which is Queen Mary's School of Biological and Chemical Sciences department. Yes completely lego, have to abit its one of the coolest buildings, or at least striking of that on campus ;) </td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEht4mOfZais0JYT0hjsorHvbYShjlMYfoIt_zHFogPDjKvahynU1-Fdx6-EcbJjbv9knzDLIepTlZfoyjCAG7A4X-Mu_qyFRApQPYjFgIyqH2UdX1OR-fGt8mVbr63pR_RIhCYGKEf2-4xr/s1600/DSC04861.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEht4mOfZais0JYT0hjsorHvbYShjlMYfoIt_zHFogPDjKvahynU1-Fdx6-EcbJjbv9knzDLIepTlZfoyjCAG7A4X-Mu_qyFRApQPYjFgIyqH2UdX1OR-fGt8mVbr63pR_RIhCYGKEf2-4xr/s1600/DSC04861.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">So we walked to the opposite side of the canal by the university to have lunch. What you observe: the blue building is student accommodation. Loving the hole in the middle of the building, in chinese feng shui terms its very good for a building to have a structured hole like that in the middle~ This is most of QM most expensive halls~ think its £180, but they get a bath tub ensuite! Posh! My first year halls is the brown halls right next to it on this right hand side of this photo. It's called Pooley house~ ensuite~ have fun memories there. Oh gosh how first year seems so ever long ago, but yet has passed so recently! </td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhDZu9VXuyEsVMd0menaClmII0sp9PWNYez0SN9_kmJCw_VAKGq76w3rDFAUpXDWJSHVIESJW0xine886K-Qkm4sKe7Bv5LpPyjNok_6u6fu9xkkosYAQC1zot2dJf49vJmaGPXBW2s5qzV/s1600/DSC04862.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhDZu9VXuyEsVMd0menaClmII0sp9PWNYez0SN9_kmJCw_VAKGq76w3rDFAUpXDWJSHVIESJW0xine886K-Qkm4sKe7Bv5LpPyjNok_6u6fu9xkkosYAQC1zot2dJf49vJmaGPXBW2s5qzV/s1600/DSC04862.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">By the canal, see this pretty canal boat with flowers on the top. Have to admit this area is a pretty nice area to relax and go for lunch on a hot summers day :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgYt38ZHosPj5R7SY01QocktqEY4k9Sa3gncnOqX8h46qDEsIvE5fR3gCn0KxLrmzdtbxQ6MNtwkY0HtHEWI7X4_TvJo3Kj9ua7yqkc-ULiqS6jbovrBxkwDzkX_sCORY6_aIN4-bTDNcvl/s1600/DSC04863.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgYt38ZHosPj5R7SY01QocktqEY4k9Sa3gncnOqX8h46qDEsIvE5fR3gCn0KxLrmzdtbxQ6MNtwkY0HtHEWI7X4_TvJo3Kj9ua7yqkc-ULiqS6jbovrBxkwDzkX_sCORY6_aIN4-bTDNcvl/s1600/DSC04863.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">My packed lunch bag. Yes it is abit childish, but I'm quite into all the cute things :) You are never too old, haha! Also, because I'm small and orential I can probably get away with it. Also, I brought a Costa (there is a Costa opposite my university) ice coffee to keep me going after lunch, especially because we have been in for so long, and had even more hours to do after ! </td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhHMXlpVgys3do7ZR99JA0RawPR0T7i6S6pWEcG1gP_ap2O6TE9sygu_FE58UD_nwluA4Y6sARxiMhf7o50gov3wnhyphenhyphenwOBTb3YrvZaFI9zN3A8acmp9mjaSycAj2Qc5B4aNNGaylwaxYH8H/s1600/DSC04864.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhHMXlpVgys3do7ZR99JA0RawPR0T7i6S6pWEcG1gP_ap2O6TE9sygu_FE58UD_nwluA4Y6sARxiMhf7o50gov3wnhyphenhyphenwOBTb3YrvZaFI9zN3A8acmp9mjaSycAj2Qc5B4aNNGaylwaxYH8H/s1600/DSC04864.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Peak into my lunch box. Love siesta lunchboxes very clever designs! Healthy food = healthy mind :) good motto!</td></tr>
</tbody></table>
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<div style="text-align: center;">
<b><u> Back to the labs!</u></b></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjy11GPzv-kPYT1NnC3uOQ2sBHIgjEXcM9vmRu9X99z8g44ZX5isW0B2QqUUEgKs81QF9ojb9hINrtN5M6aEj27vdsUBSh3yd9tf5sm9DRu_H1YFJC9Y6Dxv7rCDDBDwA84Et4KkKlb2HXL/s1600/DSC04867.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjy11GPzv-kPYT1NnC3uOQ2sBHIgjEXcM9vmRu9X99z8g44ZX5isW0B2QqUUEgKs81QF9ojb9hINrtN5M6aEj27vdsUBSh3yd9tf5sm9DRu_H1YFJC9Y6Dxv7rCDDBDwA84Et4KkKlb2HXL/s1600/DSC04867.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Really love the labs timer. Its magnetic and fits nicely into my small hands. So need one for my oven as it doesn't have a timer, this piece of tech would be extremely useful!</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEigagjgK7sAQ0s-vWlDV9A7nNSx-IPTMoWOggaMap67FFrqEgXJdjozHsK9ultfoBAADwl1RMZfAxoxj987kwwymjEywPv_02elT0y5nfyA0EtcXQ1KaGna1YSjpZxx70aEznJfg_hyqeh7/s1600/DSC04868.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEigagjgK7sAQ0s-vWlDV9A7nNSx-IPTMoWOggaMap67FFrqEgXJdjozHsK9ultfoBAADwl1RMZfAxoxj987kwwymjEywPv_02elT0y5nfyA0EtcXQ1KaGna1YSjpZxx70aEznJfg_hyqeh7/s1600/DSC04868.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is a larger centrifuge, which can spin to max speeds of 30,000 rpm! Looks like a japanese washing machine to me :P</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhLN0sM_Se0KZTNtsY1lb2UBgEA_me-_nopm7IbgKYAgzhEiHHqN6QtXqxDKpvcE4DCSA4Qz0CIrpJdhz0ORqXsv1_bFyGE-oCQdvFfEO11l1YtT29krHgx_HPpVYpRfy1_wVHblAFcdga-/s1600/DSC04870.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhLN0sM_Se0KZTNtsY1lb2UBgEA_me-_nopm7IbgKYAgzhEiHHqN6QtXqxDKpvcE4DCSA4Qz0CIrpJdhz0ORqXsv1_bFyGE-oCQdvFfEO11l1YtT29krHgx_HPpVYpRfy1_wVHblAFcdga-/s1600/DSC04870.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Because the large centrifuge doesn't have a pre cool setting, we keep the inner disc parts in the fridge to pre cool them that way!</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicLnEcJ7O-1xBxYBfdv8LQ3lspkC_oh0Sl7Cbh_yklAFut8qdPgN3CAUzuEDapguXSVFTxuk__KIij5B482t4qhBs3cKDQAGKXYx1L7yKUbW_3iz05JfPJ8BKjNWHAyaCORHjRZkvwyTLY/s1600/DSC04871.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicLnEcJ7O-1xBxYBfdv8LQ3lspkC_oh0Sl7Cbh_yklAFut8qdPgN3CAUzuEDapguXSVFTxuk__KIij5B482t4qhBs3cKDQAGKXYx1L7yKUbW_3iz05JfPJ8BKjNWHAyaCORHjRZkvwyTLY/s1600/DSC04871.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Oh how UK weather changes so rapidly. Now it is gloomy! But the nice lego architecture of Fogg building always brightens any day!</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgWw3jguNysXTBgaBHLqTUJPctd007UgvFvsGHZikpehjqaG_kk4gPsWTELnofQUzp5sMH0bHt74J8NtORMySIzBUR22u53Bcd49aDDlQZjp1UsSJ5yAkBpai19fCIp9_OioUUzXg6klMIP/s1600/DSC04872.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgWw3jguNysXTBgaBHLqTUJPctd007UgvFvsGHZikpehjqaG_kk4gPsWTELnofQUzp5sMH0bHt74J8NtORMySIzBUR22u53Bcd49aDDlQZjp1UsSJ5yAkBpai19fCIp9_OioUUzXg6klMIP/s1600/DSC04872.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This looks like it could be part of a canon! It is where we place out test tubes for centrifugation.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhl8L9bLd_mb3E9LbHBo_eb3Io9YYJxcE6tMqcw0Fl_hjV8Y3R1NsRXYENlv6-sZHDX9n63KzHvFLHUSSV_FzrhyBAqMGe-Uto8AOr-9k_XvSp1x9Y6m2v_BOLJufjdJabjW-68zhwMwnu8/s1600/DSC04873.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhl8L9bLd_mb3E9LbHBo_eb3Io9YYJxcE6tMqcw0Fl_hjV8Y3R1NsRXYENlv6-sZHDX9n63KzHvFLHUSSV_FzrhyBAqMGe-Uto8AOr-9k_XvSp1x9Y6m2v_BOLJufjdJabjW-68zhwMwnu8/s1600/DSC04873.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Massive microwave! Well looks like this, this moves whatever you put in it in a square motion!</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi6ZulcMJ9tjXPeXh9f1BTdBCocoS-Hm3YvlTgRFzy0slKaAu8LsWGe7OR0gi7XHH6ZLbZE5PUkZxuKvZleStBIRFSpFcU0UVGdKa5dpT2fH4_h3ObsjaoZWc1QSiuoZWvXdysnIr1fQ5LD/s1600/DSC04874.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi6ZulcMJ9tjXPeXh9f1BTdBCocoS-Hm3YvlTgRFzy0slKaAu8LsWGe7OR0gi7XHH6ZLbZE5PUkZxuKvZleStBIRFSpFcU0UVGdKa5dpT2fH4_h3ObsjaoZWc1QSiuoZWvXdysnIr1fQ5LD/s1600/DSC04874.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Placing in the fridge already pre cool disc part of the centrifuge.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgdzvvGnW2vCcTZItQN5-d4Lw93VnIuA50L711pdCQ9z-r7W1YwDK4gci-TsKzdgImfvXWB0luRD-nqz6l1frPq3_3O79woxboCG_8Va5Z9Li_rkVYMNy5vXPCptGBNH93cXJonIvsBjEWr/s1600/DSC04875.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgdzvvGnW2vCcTZItQN5-d4Lw93VnIuA50L711pdCQ9z-r7W1YwDK4gci-TsKzdgImfvXWB0luRD-nqz6l1frPq3_3O79woxboCG_8Va5Z9Li_rkVYMNy5vXPCptGBNH93cXJonIvsBjEWr/s1600/DSC04875.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Fixing on the lid.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYmPDF7l5GO-lZDvB2ze_TEhyoJ9BFcSg_yFunX3sU7Tp1aP8xaXvH5ntafYsh1XGo_1BUTxXH5Oi6Pju78v_RoNs4cTTOfUTUi6K1B-hg2AbpvZmLgeeif8L4s2COwNfwiP0YMdZ_OncJ/s1600/DSC04876.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYmPDF7l5GO-lZDvB2ze_TEhyoJ9BFcSg_yFunX3sU7Tp1aP8xaXvH5ntafYsh1XGo_1BUTxXH5Oi6Pju78v_RoNs4cTTOfUTUi6K1B-hg2AbpvZmLgeeif8L4s2COwNfwiP0YMdZ_OncJ/s1600/DSC04876.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Seriously looks like a japanese washing machine...</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg3gIAS5q-uq2rKC19AwaI3xfJwLPztCuapfnMyt0lmQatn0hVL5SbqJjL-asozAtaAfJ5G3F0fthI0cZiqQERpeACEjNKG418jTwCthJAOeXhVSjexDlseBhaBi-QcXxYwRQLWuUyS2W0E/s1600/DSC04877.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg3gIAS5q-uq2rKC19AwaI3xfJwLPztCuapfnMyt0lmQatn0hVL5SbqJjL-asozAtaAfJ5G3F0fthI0cZiqQERpeACEjNKG418jTwCthJAOeXhVSjexDlseBhaBi-QcXxYwRQLWuUyS2W0E/s1600/DSC04877.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Adjusting the settings.</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-xfOXyzCVLmCyZM9CQ4Z0IMA61QjtviEn3wp4qPxPCQualePlGgkwQr4snECBai82BsYCvyl-ZvMzKfHUJCwp02w0b0mBWrm-1OurDyhiWbPda-UUAW0YdOxuo5vengG_A_Fah5uz6l28/s1600/DSC04878.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-xfOXyzCVLmCyZM9CQ4Z0IMA61QjtviEn3wp4qPxPCQualePlGgkwQr4snECBai82BsYCvyl-ZvMzKfHUJCwp02w0b0mBWrm-1OurDyhiWbPda-UUAW0YdOxuo5vengG_A_Fah5uz6l28/s1600/DSC04878.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here is is in lab.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiGXXu-FmFKe_nQHyMX5_-yvrszTFs680hyG9h-y2QyKAhgWT6SJxEHI63UiNC0fI0FX_kfgaF-J95Rxuzw01wcAMMD39Gl68KMIwKqptMZi-cziYuiH4GOEXOfeRjBHZbOjgtnLfPnXDHT/s1600/DSC04879.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiGXXu-FmFKe_nQHyMX5_-yvrszTFs680hyG9h-y2QyKAhgWT6SJxEHI63UiNC0fI0FX_kfgaF-J95Rxuzw01wcAMMD39Gl68KMIwKqptMZi-cziYuiH4GOEXOfeRjBHZbOjgtnLfPnXDHT/s1600/DSC04879.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Ruban's office is very close to the large centrifuge, so I thought to take a photo :) Where all the meetings are!</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjv8boudsjXkjFdadOIJ-Jlu6jFLpab4g5lrt1oj_6EcRCtkgeo76AdycLcZE98WcuooRtUxK7-n9TXlrjP7NYgFlBP9DTe4BCmV9Hn29llDrHakMRI4XBsMaQmOe1cnw6ArhnHrAcCG1cm/s1600/DSC04880.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjv8boudsjXkjFdadOIJ-Jlu6jFLpab4g5lrt1oj_6EcRCtkgeo76AdycLcZE98WcuooRtUxK7-n9TXlrjP7NYgFlBP9DTe4BCmV9Hn29llDrHakMRI4XBsMaQmOe1cnw6ArhnHrAcCG1cm/s1600/DSC04880.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Very concentrated material!</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiPehSJxyGKY2bM0M6b_qjXcOkFQUlXLiQ_xzhbu1GNleYfqlZEjjS0cmZncVoDSq7R0ZcEBoKC5ZqzAu1kmccEUgJ0mTWbIZWDD9rCkqNvT7E_oYZnTCRcgt9qIFMveM5FKPuGH11_HhHT/s1600/DSC04881.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiPehSJxyGKY2bM0M6b_qjXcOkFQUlXLiQ_xzhbu1GNleYfqlZEjjS0cmZncVoDSq7R0ZcEBoKC5ZqzAu1kmccEUgJ0mTWbIZWDD9rCkqNvT7E_oYZnTCRcgt9qIFMveM5FKPuGH11_HhHT/s1600/DSC04881.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Me using a 5ml pipette, look so ready in action.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg0jhGi4nCMVtLkdoa-x4Wgcc2JBsGqlq-ZszAtBw_cpfQZkozqU7ej66mhRTgW8rcS0c8pfOsGQdEQZnIR33NcfaGQtdGDHlRGsRmFrhWC7n-z9u9xPkRAYxPFI8Eh5AGg4naZxwAg0orn/s1600/DSC04882.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg0jhGi4nCMVtLkdoa-x4Wgcc2JBsGqlq-ZszAtBw_cpfQZkozqU7ej66mhRTgW8rcS0c8pfOsGQdEQZnIR33NcfaGQtdGDHlRGsRmFrhWC7n-z9u9xPkRAYxPFI8Eh5AGg4naZxwAg0orn/s1600/DSC04882.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Inverting the tube to ensure uniformity in solution.</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgCUuwEv4MRvPU3hKJLgbPvmEcuvVWpPaJxI8h58t1T50In8ffy6CEhGArP1BvvVcKuHIAo0kcwJBpxjc3NAZB_7AICmcmYPGd5AFEo4GHWljbbroOIRkffbinnQWqrLeYr_D4A87zyjZgk/s1600/DSC04883.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgCUuwEv4MRvPU3hKJLgbPvmEcuvVWpPaJxI8h58t1T50In8ffy6CEhGArP1BvvVcKuHIAo0kcwJBpxjc3NAZB_7AICmcmYPGd5AFEo4GHWljbbroOIRkffbinnQWqrLeYr_D4A87zyjZgk/s1600/DSC04883.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">take #2 : smiles :D</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgZZNYf3O1-jrj6f-QlDCWKXZo4KaLAgPpWKgoeZNUNMaAWDxM3GlOWzNq33ORQz4pZnbG0Bqfx4HQtoG9Ktn18jGpaddwx3wRlianZO1nJKTHYGhnbBG_4Whfysz7HtsVhvrC6QqBwZRDF/s1600/DSC04884.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgZZNYf3O1-jrj6f-QlDCWKXZo4KaLAgPpWKgoeZNUNMaAWDxM3GlOWzNq33ORQz4pZnbG0Bqfx4HQtoG9Ktn18jGpaddwx3wRlianZO1nJKTHYGhnbBG_4Whfysz7HtsVhvrC6QqBwZRDF/s1600/DSC04884.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Counter balance of water, checking via eye that it is roughly the same in level. Obviously, we measured it out before hand in weight to ensure it is correctly balanced.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Placing the tubes in!</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Signing in that we used the centrifuge last. </td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjJgXrJ_xD8n7VOf-7IFUR-qUIQoFfAOY560t_4r9m6HXpXOhCAQWizn1faaZmIxSBBBwye13akMRfV9YuFJRL2DEctS-IWJ9gzkHZaC4GYJqentP8Czs9laOc37lblPQ1sOl3UYZLy9gdc/s1600/DSC04890.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjJgXrJ_xD8n7VOf-7IFUR-qUIQoFfAOY560t_4r9m6HXpXOhCAQWizn1faaZmIxSBBBwye13akMRfV9YuFJRL2DEctS-IWJ9gzkHZaC4GYJqentP8Czs9laOc37lblPQ1sOl3UYZLy9gdc/s1600/DSC04890.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Using a paint brush and added H20 to re suspend the pellet obtained from the large centrifuge, important to keep in icy temperatures as mentioned before!</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Using a syringe to filter through the material through a mini filter placed on top of the ependorf tube.</td></tr>
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<b><u> End of the day: Autoclave time:</u></b></div>
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<tr><td class="tr-caption" style="text-align: center;">So there is a trolley outside the autoclave room which all the labs on the fourth floor place their equipment on, which they desired to be autoclaved.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Placing all the equipment in the trolley in the autoclave! </td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgzrgqB4MR6eyS_xV04eXkz2RFnv683rzHWbFYHMNoXWrX8o-ab2H3TJwNDwcePW65Tskt6b_oKxWJvqlsix9RoPk51tDKTe4kLfCIp61dEODKC6hLFWEa9fpb_I_XAwW8aMNXa-93T6XOB/s1600/DSC04900.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgzrgqB4MR6eyS_xV04eXkz2RFnv683rzHWbFYHMNoXWrX8o-ab2H3TJwNDwcePW65Tskt6b_oKxWJvqlsix9RoPk51tDKTe4kLfCIp61dEODKC6hLFWEa9fpb_I_XAwW8aMNXa-93T6XOB/s1600/DSC04900.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Autoclave in a different position. Seriously looks like a massive pizza oven! I swear I always think that everything in the lab looks like something else in life, haha.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">The instructions for which setting to set for what you are autoclaving. Pretty self explanatory.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Everything set. READY TO GO.<br />
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<b><u>Finally, the end of the day: </u>What a long a day! </b></div>
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And also a blog post too! Haha hope you enjoyed it :)</div>
J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-87803460788752739832014-06-11T20:00:00.000-07:002014-07-01T08:54:24.927-07:00Third Repeat: Isolation of Stacked Thylakoid Experiment<div class="separator" style="clear: both; text-align: center;">
So this was my third time of repeating the stacked thylakoid protocol, changing the parameters yet again.<br />We had a sample loop of 300ul for the injection value (smaller the sample loop the greater the resolution of our FPLC curve separation)<br />Also, we used a higher alpha DM detergent concentration of 1.2%<br /><br />Due to the fact this was my third time doing this experiment, I was pretty rapid so had more times to take random snaps around the lab. Quite unfortunate as I actually forgot my camera on this day, oh well my camera is pretty decent with its 8megapixels:<br /></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEifeYRtvNfmkpX_jpw5XGSWgiFB-69JsjKRgB5yH5VSNpDPri-IO5j-OBFAY58tf0cl1zPqn9hSzy055jJSyl7oUsL1tlFDCLMrjOtjlACa4XlH5JXVfCdHgZdraXxQlPcIpN16iAvxjQv5/s1600/IMG_20140618_113841.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEifeYRtvNfmkpX_jpw5XGSWgiFB-69JsjKRgB5yH5VSNpDPri-IO5j-OBFAY58tf0cl1zPqn9hSzy055jJSyl7oUsL1tlFDCLMrjOtjlACa4XlH5JXVfCdHgZdraXxQlPcIpN16iAvxjQv5/s1600/IMG_20140618_113841.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">One of Ruban's lab, the other one being a dark adapted room!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj3Q1SVTISNB70hFMOmoM9B84bNZ8CeJiDNwAJbk2GLnSSVUu81aKp3Sx_V3KoUouiAIJPgjAj8oZzWF6gEACOTT00HHL3LOIEEicRjtD5Ag0AKii9ebQX0z-FjMMEYVuwTKARSTz2kr3bF/s1600/DSC04664.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj3Q1SVTISNB70hFMOmoM9B84bNZ8CeJiDNwAJbk2GLnSSVUu81aKp3Sx_V3KoUouiAIJPgjAj8oZzWF6gEACOTT00HHL3LOIEEicRjtD5Ag0AKii9ebQX0z-FjMMEYVuwTKARSTz2kr3bF/s1600/DSC04664.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">While waiting for the light treatment to occur on the spinach leaves, Petra made us do calculations to work out the correct mass of each chemical we needed. It was already on the sheet, but it's always good to get practice. I always hated these in lab practicals as they always confused me, but have to say they are not just something the lecturers make you do for nothing! They are extremely useful in the working life of a scientist!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjDsw16pRqvL4RjOgwy2Ys6y2GQGjix7kU3ptCvvGKEjM52viZhlFMR59DiWhUt_H9D0XGUdjvx3gTenTW26YKVfi4wYZPMOmnUXViGy_TnEkYdPWk8d_uQ_DbT-mg8TmfMtLTd2dmh-Vsw/s1600/IMG_20140618_105342.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjDsw16pRqvL4RjOgwy2Ys6y2GQGjix7kU3ptCvvGKEjM52viZhlFMR59DiWhUt_H9D0XGUdjvx3gTenTW26YKVfi4wYZPMOmnUXViGy_TnEkYdPWk8d_uQ_DbT-mg8TmfMtLTd2dmh-Vsw/s1600/IMG_20140618_105342.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">After using the polytron to grind up all the spinach leaves in a uniform liquid, filtering through the layers of muslin; getting rid of the large starch particles in the spinach leaves we do not desire. Ohhh so much green! :3</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjTVOr7MzDtFR2szCsL3p1a0FfzgD9yYN5P0ned1n_8YB1Si_YEUkN6B_TvQAbsdV-zrGZ5joVTf7tNfm8TqiLXJyIvMF00PH9OwdvPRpbvapvrFxyNx4SSkEHc22eeYR0GuDEidshGT0KD/s1600/IMG_20140618_111148.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjTVOr7MzDtFR2szCsL3p1a0FfzgD9yYN5P0ned1n_8YB1Si_YEUkN6B_TvQAbsdV-zrGZ5joVTf7tNfm8TqiLXJyIvMF00PH9OwdvPRpbvapvrFxyNx4SSkEHc22eeYR0GuDEidshGT0KD/s1600/IMG_20140618_111148.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Always refering back to the lab book and my little written notes! See my pencil case? Very tempted to get a large see through one that can fit in all my highlighters, pen, pencils, calculators, rulers...as currently everything chucked into my hello kitty pencil makes it a "fat hello kitty" :( Generally don't like the bulkyness in my bag. Also, clear through pencil cases means I don't have to mess around with removing my pens for the exams~ I can just take the whole thing into the dam exam! :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiOnMf4yWa0UXw1pvLWiPEnnFb28ugWXqnjm-bp5mJyt-SpRZqUXsJiTgxh5Dyq-DULlDQufOkf0GgyXQCdqpxZ_6aa3n8pu2MW50k3ZDoDecmIzxJ8QsLtFoCvJCYwOJi9wdR_iVtpA2OX/s1600/IMG_20140618_111202.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiOnMf4yWa0UXw1pvLWiPEnnFb28ugWXqnjm-bp5mJyt-SpRZqUXsJiTgxh5Dyq-DULlDQufOkf0GgyXQCdqpxZ_6aa3n8pu2MW50k3ZDoDecmIzxJ8QsLtFoCvJCYwOJi9wdR_iVtpA2OX/s1600/IMG_20140618_111202.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Everything must be kept on ice!!! Preventing any biochemical reactions from occuring. Oh look the ice even started to turn green! Haha! (I think it was from a minor spillage)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgxYdKooNd2eoieMJEqHz3iAUjZYZEefFY9YfcARUVUOu9U7KZoLekpL79KTr8QlWLbjss-05D9YHtalUT2TihaSyB-5ZHQgM58LpzTqZQRx0TFKcdSHW840pNFQ0dBQpKj6mkv7Cf43kZG/s1600/IMG_20140618_123757.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgxYdKooNd2eoieMJEqHz3iAUjZYZEefFY9YfcARUVUOu9U7KZoLekpL79KTr8QlWLbjss-05D9YHtalUT2TihaSyB-5ZHQgM58LpzTqZQRx0TFKcdSHW840pNFQ0dBQpKj6mkv7Cf43kZG/s1600/IMG_20140618_123757.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This machine spins the box in a square manner for the amount of time we desired. This is the part which we call the dark treatment. We usually, after setting the spinning speed place a black cloth over our ice box with our material in, to aid the dark conditions, for treatment.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Bag full of micro centrifuge tubes!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgPiO8PkXxI4BCzITgg5fjhNZ_BQXZ_HRiaios2sOWyZ9RoDN-E5i1PtsEKNsV5oOq05ZqT-ar1mxmcgEL_9jNxdc4SZwdEQGGDNqB4WCnWWUENe3DKCNW7AqPdI63AwKjKnj45l6Cuo6F5/s1600/IMG_20140618_124207.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgPiO8PkXxI4BCzITgg5fjhNZ_BQXZ_HRiaios2sOWyZ9RoDN-E5i1PtsEKNsV5oOq05ZqT-ar1mxmcgEL_9jNxdc4SZwdEQGGDNqB4WCnWWUENe3DKCNW7AqPdI63AwKjKnj45l6Cuo6F5/s1600/IMG_20140618_124207.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here they are hidden in the draw~</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjtSJ0LNQ1XG1uuTs1do5Hx7vayLBsoZYOHXuein5SOdqSNg7k05McF6wA6GqOZDlNwFQpydKtV7p13d0igfjMtD0vrHFUs5YvMixWmtVZ0YAqKDyjKqTOVAtLOgdjZDU_B5toiPtBywKJU/s1600/IMG_20140618_124403.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjtSJ0LNQ1XG1uuTs1do5Hx7vayLBsoZYOHXuein5SOdqSNg7k05McF6wA6GqOZDlNwFQpydKtV7p13d0igfjMtD0vrHFUs5YvMixWmtVZ0YAqKDyjKqTOVAtLOgdjZDU_B5toiPtBywKJU/s1600/IMG_20140618_124403.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Vivid colours in the lab~ always good to add colour to your life. Especially in the lab its always ever so grey in that room! Here I am adding up my O/D for chorophyll A and B measurements in the spinach leaves.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Syringe, which we use to load our sample into the 300ul sample loop at the injection valve!<br /><br /></td></tr>
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<b>Then we ran FPLC to separate our fractions! </b></div>
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However, this time of stacked thylakoid preparation, Erica (The postdoctoral researcher at Ruban's Lab) allowed us to analyse certain fractions we obtained from FPLC via a method called 77k (using a machine called fluoromax-3). This method allowed us to determine exactly what was in our fractions, as before we were estimating from previous graphs as we knew what peaks would give what complexes. Also, it allowed us to see how efficient our preparation in stacked thylakoids were in providing us with our desired PSII super-complexes only. All this was performed in the dark room (Ruban's second lab)!<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjiclC5X_A8SOn49_I3ywl-qXDJT7T8igQ1LccsdEDTu9jQJ8ainT2SDG9myU0rkNYQKA5DJyZDtB5URNGQzmfoL-S7je4-VveQGO3nzVtkrjLF4GpQlrPvxDuPds0SDZE90Pk2SwXO4oXY/s1600/IMG_20140618_143120.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjiclC5X_A8SOn49_I3ywl-qXDJT7T8igQ1LccsdEDTu9jQJ8ainT2SDG9myU0rkNYQKA5DJyZDtB5URNGQzmfoL-S7je4-VveQGO3nzVtkrjLF4GpQlrPvxDuPds0SDZE90Pk2SwXO4oXY/s1600/IMG_20140618_143120.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Always keeping our fractions on ice prevent the reactions !</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjpyYyY2_-JS3Yf1-YitpH6c-1mcAfG3g1cOMDr837Tm3bsQkEQR6XzTTYj-fcTGfpdxfdIiL58W4VWlsTsWXHwZ1lLA6_chr_9U2t_BN1SMha4ssGWsWpG4iBUhSyDTDWLqhlEROV7kwSs/s1600/IMG_20140618_143433.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjpyYyY2_-JS3Yf1-YitpH6c-1mcAfG3g1cOMDr837Tm3bsQkEQR6XzTTYj-fcTGfpdxfdIiL58W4VWlsTsWXHwZ1lLA6_chr_9U2t_BN1SMha4ssGWsWpG4iBUhSyDTDWLqhlEROV7kwSs/s1600/IMG_20140618_143433.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">As you can tell from the illumination from the computer screen, we are in the dark room. The 77k part is the white cylinder that Erica's hand is placed around. She is adjusting the sample to allow the florescence to hit our sample in order to detect what is actually inside! </td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Adjusting the programmes parameters to get a clearer scatterpatch which we can use to determine the exact complexes in our fractions.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">A black cloth over the 77k part of the fluoromax-3, aiding the conditions even more, even though the machine is in a dark room already and lights are switched off!</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">The name.</td></tr>
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To conclude, what we found from the 77k is that our A9 fractions which are meant to contain high amounts of PSII supercomplexes, still contain a high amount of PSI supercomplexes. Therefore, we know for sure that the stacked thylakoid preparation is not suitable for what we desire- isolating only PSII supercomplexes so we can analyse the efficiency of Violaxanthin to Zeaxanthin of the xanthophyll cycle, which occurs when the plant is in high light stress.<br /><br />This leads to the fact we know now to use the BBY protocol, which is a preparation very similar to that of isolation of stacked thylakoids, however much longer as more steps are involved. However, at least with BBY protocol obtaining only PSII super complexes is the standard outcome.<br />
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<br />J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-78456776601223555862014-06-06T16:00:00.000-07:002014-06-20T04:16:21.295-07:00Analysis of HPLC results<div class="separator" style="clear: both; text-align: center;">
I had arranged to meet a friend after lab yesterday! Yes work hard and play hard ! Totally live by that motto~ as life is too short to 100% dedicate yourself to just work only. You need to enjoy yourself once in a while. There is more to life that just lab, university~ as much as I love learning and science. I am really big on being social and outgoing :)<br /><br />So unfortunately didn't have the time to analyse, annotate and stick in my results into my beloved lab book. So I'm doing it this evening! Here are photos to show you a glimpse of what it's like to be in Jessica Lau's work space~ haha!</div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhimpFcZjPuhyt7BojgNCkuYLiaWGeRgMlBPQBN5zOQ29RDum3LGhrJunvnLCgWGfLVbT4gt7SA0hrJz_JfAMUPm3Yju6SXQUsrOpDiXJ412Tr_i71TheTtuZURlzo-IBQhIp_bcoJNTx5x/s1600/DSC04625.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhimpFcZjPuhyt7BojgNCkuYLiaWGeRgMlBPQBN5zOQ29RDum3LGhrJunvnLCgWGfLVbT4gt7SA0hrJz_JfAMUPm3Yju6SXQUsrOpDiXJ412Tr_i71TheTtuZURlzo-IBQhIp_bcoJNTx5x/s1600/DSC04625.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is my study area in my room, complete with my whiteboard for writing ideas, revision etc~ I recently brought the exam desk table from amazon to make more study place. Before it was just the white corner desk that existed and I didn't really have enough study space, with having all the books, laptop, printer, notes out. I just like having easy access to everything, so this exam desk, which was only £15 was a great investment! I did consider getting a new desk in all but I really like the corner desk for storage and it fits nicely in the corner! I'm quite a interior designer, space saving idea filled individual myself. One of the best dates for me is if a guy took me to Ikea! Hahah~ I know you should never wish away your life but I actually cannot wait till I'm older, finally starting my career in the science world, where I can have my own place to decorate and make my own ^_^<br /><br />Also with this new addition of an exam desk, I can shove all my boxes of notes underneath the corner desk as pictured, which before these boxes use to cover up a beautiful fireplace in my room! So win win! I am a true believer that you really should make your "study area" your own as studying is a happy thing :) Well for me anyways. I have inspiring notes on the walls of mine to keep me going through the tough days. Maybe I'll make a blog in the future about how I personally keep on studying for long hours and how the study area is my own place of zen! Yes, anyways so you can tell I've made this area a place of my own with my monster inc poster and cuddly toys~ which personally make me really happy~ you all should do too!<br /><br />Lastly,the addition of a corkboad means I can pin important notes up rather than sticking them up with bluetack, which i really like! It's just easy to pin and its right infront of me for future references!</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Printed all my results out! Now do you see what I mean that I have a larger study space surface area! It's great spreading everything out, being able to see everything! Before it was a real up time struggle and I hated putting things on the floor. I did try and get chairs from the dining room table and put the notes on that, but still they weren't in easy view of being on the table!</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Nicely edited my results and compacted them so they would be easy to stick and read in my lab book!.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Close up on the graphs generated from HPLC~ seeing clearly what pigments we have. By calculating the area underneath the pigments you can obtain the amount of pigments in the complexes. There is already a generated excel file which we can place our values in which calculates it for us. Thanks to the scientist that worked before at Ruban's lab; making my life easier! Gosh, imagine doing all the calculations by hand, would take at least an hour!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhubMWYPzwEeL_IqOCO3T8ASJvT1Mb-2ACVju3KVJ0CsmSWdLEhbnzdsUD2bZRe-Eu-5YjSxrM-CDKYjbw9cgDIPbFhoDBKjr6fYW6Q5NOluZOoojFktXvq7Y-mOsVPwAnNGxs0VLu0D8Qy/s1600/DSC04663.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhubMWYPzwEeL_IqOCO3T8ASJvT1Mb-2ACVju3KVJ0CsmSWdLEhbnzdsUD2bZRe-Eu-5YjSxrM-CDKYjbw9cgDIPbFhoDBKjr6fYW6Q5NOluZOoojFktXvq7Y-mOsVPwAnNGxs0VLu0D8Qy/s1600/DSC04663.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here's the results stuck in~ analysis with a lot of highlighters, pens, sticky notes~ Yes I am really big on colour. I think it's quite obvious from my study area in the room, it's quite colourful compared to a plain library study area. Colour excites my brain and helps me to visualise and learn~ I've always been a big visual learner :) Also it makes my beloved lab book (Yes I am dearly in love with my lab book because I've put so much time into it) so pretty to just view!</td></tr>
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Anyways~ Analysis of results should usually be done quite close to the experiment as you don't want to forget important information! If I have the time usually I prefer doing everything in the lab~!<br />
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J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-41434876311654568892014-06-05T20:00:00.000-07:002014-06-20T03:53:26.838-07:00UK’s Beautiful Sunshine Rays + First Run of HPLC<div class="MsoNormal">
Today was my first time on the Fogg's rooftop (Fogg
Building= Queen Mary’s School of Biological and Chemical Sciences building). It’s
basically where all the greenhouses are for the school. Here are some lovely
pictures of it:</div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEia7-fW8kFJhu5NmngnCHkzkPNkWbk6DaDOTe8-zfOw4bXj58OP_LOmbDFcu_64X6P0XZTeDhHIhFu_ygSKtkuYDFGClsUpjINE3pH3YMK1iecSPSbQp9ep0mLOo9ugSyiG4zhGp7tJFjww/s1600/DSC04581.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEia7-fW8kFJhu5NmngnCHkzkPNkWbk6DaDOTe8-zfOw4bXj58OP_LOmbDFcu_64X6P0XZTeDhHIhFu_ygSKtkuYDFGClsUpjINE3pH3YMK1iecSPSbQp9ep0mLOo9ugSyiG4zhGp7tJFjww/s1600/DSC04581.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here are the several green houses on the rooftop. If you can see the blue door in the picture, there are more on the other side of the door!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjXOxOe5wgRrHOBc7rXHYq_zbomIRx1x1TxDHMB99R2l0t-5R3lLt8FL4tn2aL9ZJjzfOp5u9NXC7qMEqGdpnPLnjv92_RcNt6wGZlz7ykjxf5LgJzDE2iXmh-ND3bRfWjZW5sIm2kvbbj5/s1600/DSC04582.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjXOxOe5wgRrHOBc7rXHYq_zbomIRx1x1TxDHMB99R2l0t-5R3lLt8FL4tn2aL9ZJjzfOp5u9NXC7qMEqGdpnPLnjv92_RcNt6wGZlz7ykjxf5LgJzDE2iXmh-ND3bRfWjZW5sIm2kvbbj5/s1600/DSC04582.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Green house shot 2# : As you can tell the green houses are quited dated and I believe money should be spent on making them nicer! Apparently when a glass window falls or breaks, inevitable when the greenhouse is that old, it costs so much to replace just the glass, so why not replace it all? Guess with most thing its about funding! Currently Fogg building is undergoing alot of work inside with refurbishing and building new offices~ so guess that's where the money is going !</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj0jxegTGDGkjzhfqtZY93rHGNVmx2-6rK4uOaghp4P04tf1-D3kx-nQkrSWiVyX9WYdsK6JYusx4xhVn4DV98KwlawHEdf1UdH1pexuqkYvmq2VjvMbvx-HPqZ4M3a-cFE9UNivY2aY_Lo/s1600/DSC04583.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj0jxegTGDGkjzhfqtZY93rHGNVmx2-6rK4uOaghp4P04tf1-D3kx-nQkrSWiVyX9WYdsK6JYusx4xhVn4DV98KwlawHEdf1UdH1pexuqkYvmq2VjvMbvx-HPqZ4M3a-cFE9UNivY2aY_Lo/s1600/DSC04583.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Loving the clouds in this picture, and you can just tell by the building that this is iconic East London!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh0HXuSe90t8kA7OmfNhFgqyi3hBseo2osyQPpWQRuqnjnBhgtiFI4d8Sw4SeUJwal8gXgocIiuLW-E6VRcsKFbNyQ5y6XHMZiHZZvMJnQQz_kYRtXsNFqXa7e42xsy4AEHC5RCX7r8XDMF/s1600/DSC04584.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh0HXuSe90t8kA7OmfNhFgqyi3hBseo2osyQPpWQRuqnjnBhgtiFI4d8Sw4SeUJwal8gXgocIiuLW-E6VRcsKFbNyQ5y6XHMZiHZZvMJnQQz_kYRtXsNFqXa7e42xsy4AEHC5RCX7r8XDMF/s1600/DSC04584.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">East London view~</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhHkEFlmQqLndtRgDvjFWMy2qugK2y1c1dUdCiyD4UuRTJg-vAJs9hUMTYArqDcekRldpopvK4Cmq4WT991lM2BrHBUGsJtgbat1KvDCzRO6kRJNWTmsu-AwMs8gMqwrfTMtAy_BSagkqI4/s1600/DSC04585.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhHkEFlmQqLndtRgDvjFWMy2qugK2y1c1dUdCiyD4UuRTJg-vAJs9hUMTYArqDcekRldpopvK4Cmq4WT991lM2BrHBUGsJtgbat1KvDCzRO6kRJNWTmsu-AwMs8gMqwrfTMtAy_BSagkqI4/s1600/DSC04585.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">A photo of the rooftop, East London views and the pathway leading back to the fifth floor of the fogg building with the ladder steps. As you can tell the Fogg building is very peculiar in itself for the fact it has this Lego theme going on it. I'll blog about the architecture of the actual Fogg building some other blog post! So look out for it in the near future!!!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhO32DLwyaulbBe6QFeqbtdxzDM20fnXAhbKSqf3Lx7VATkIIgAsMamOu-yjpY96QQUesXPuVOrKvQSw8s5RPmp7r1Whv6qi8cC5ePZeNVdqaVcEjG0WGVAGlso6hfrzzV-K_SKrTrlVhyR/s1600/DSC04586.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhO32DLwyaulbBe6QFeqbtdxzDM20fnXAhbKSqf3Lx7VATkIIgAsMamOu-yjpY96QQUesXPuVOrKvQSw8s5RPmp7r1Whv6qi8cC5ePZeNVdqaVcEjG0WGVAGlso6hfrzzV-K_SKrTrlVhyR/s1600/DSC04586.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Better view of what I described the photo above!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhxQfZlVMaBJD3Iw9X0c0Ep3NyDh8gYeD4ry6cxbAUk8QEuctGjScbc0RNyThAV6fNBCyDpvdNDci0Ey7UFgzQrZae69_GG8NtZUOc6g3S4t9aeFhAhPgGmRdXjpAynQQ_8csCulD_Vhsn-/s1600/DSC04587.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhxQfZlVMaBJD3Iw9X0c0Ep3NyDh8gYeD4ry6cxbAUk8QEuctGjScbc0RNyThAV6fNBCyDpvdNDci0Ey7UFgzQrZae69_GG8NtZUOc6g3S4t9aeFhAhPgGmRdXjpAynQQ_8csCulD_Vhsn-/s1600/DSC04587.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Petra (Ruban Labs technician) giving me a tour around Ruban Lab's Greenhouse. Ohhh looking at this picture reminds me of how amazing the weather was that day! ^^</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgr1nMMNRCmfn3G1wnVEvbrm_ObrPBaBCVmPalzFbT0wqzT3XOH2imPThK4-McQ-irbmgjSbNU13TC0JEOEUaPHYivxCMRqzDnZmuG4EusJXnCaCs8N7CqvsOOHJ6p2vJEDBt723dkSpL1G/s1600/DSC04588.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgr1nMMNRCmfn3G1wnVEvbrm_ObrPBaBCVmPalzFbT0wqzT3XOH2imPThK4-McQ-irbmgjSbNU13TC0JEOEUaPHYivxCMRqzDnZmuG4EusJXnCaCs8N7CqvsOOHJ6p2vJEDBt723dkSpL1G/s1600/DSC04588.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Botanist playarea! Haha</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjAzIQupmVVAdBnval8OmOQToxbftmzpmBshDJcG4m1T755TGDtogF5gk_mhHgLOQmBw1MXPxSkZGRXKhBi9Um5Ou9FSHHnY51wWkZNFKveM6YdmouDimtoDZ2yhxwtM025R3D2S4UL1HmR/s1600/DSC04589.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjAzIQupmVVAdBnval8OmOQToxbftmzpmBshDJcG4m1T755TGDtogF5gk_mhHgLOQmBw1MXPxSkZGRXKhBi9Um5Ou9FSHHnY51wWkZNFKveM6YdmouDimtoDZ2yhxwtM025R3D2S4UL1HmR/s1600/DSC04589.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Anna's Plant. Anna is a final year Phd student of Ruban's. She works on hybrid plants! Frankenstein like she says it!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEikvwZ4EnBBJ26yLcmuUHnNLYS232wRRQg5SE8p1C6BWuyE2IolTYo3qJoipkbXYk75D0xhQf57BjijLKpfuQTHv4xZ-iyq2vgORr0j_p1Ga6uKMs6sR3c8SRYWzs4vjMoqxCjPgrlvPyg1/s1600/DSC04590.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEikvwZ4EnBBJ26yLcmuUHnNLYS232wRRQg5SE8p1C6BWuyE2IolTYo3qJoipkbXYk75D0xhQf57BjijLKpfuQTHv4xZ-iyq2vgORr0j_p1Ga6uKMs6sR3c8SRYWzs4vjMoqxCjPgrlvPyg1/s1600/DSC04590.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Healthy plant growth</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgajDUBZ9-30Z1sWQ8CGlVsWZJkfsx4Dax5Gx-gqiNdBT17FPHLqyyGdPRCo6bdFIbOOQzliGoBg_CY1FaaqnwUn9a-ljb0o3SuWkuTTPzyt3eXImfn3XZd-aSlCimUmHlwYXnrCW7ZeOP0/s1600/DSC04591.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgajDUBZ9-30Z1sWQ8CGlVsWZJkfsx4Dax5Gx-gqiNdBT17FPHLqyyGdPRCo6bdFIbOOQzliGoBg_CY1FaaqnwUn9a-ljb0o3SuWkuTTPzyt3eXImfn3XZd-aSlCimUmHlwYXnrCW7ZeOP0/s1600/DSC04591.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">They look like they are dying but this is how we obtain the tiny seeds from them! Where Petra hand is positioned is basically where all the fine seeds are located.<br />
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<span style="font-size: small;">We started off the lab day
discussing about the fine details from my previous meeting with Professor Ruban
and how we can further change the parameters to improve our solubilisation for
PSII complexes. After this, we headed back down to the fourth floor, where
Ruban's Lab is.</span></div>
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<span style="font-size: small;">Finally the day had came and it was HPLC time! I’ve never
ever used a HPLC machine in my life! Since the 3 weeks of being here I've always anticipated that we would eventually use this to further analyse certain fractions obtained from FPLC. I’ve
always wondered how this machine works, how we would use it! New experiences are always the most exciting.</span></div>
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<span style="font-size: small;"><br /></span></div>
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</span>
<div style="text-align: left;">
<span style="font-size: small;">I must say, I really do prefer the HPLC machine
over to the FPLC. I find HPLC machine more compact and nicely in place. The
FPLC for me feels abit large and awkward in the sense you have the fraction collector part of FPLC. To be honest, maybe it is the positioning of FPLC, as it is placed awkwardly next to a beam that makes it so awkward to use, especially when you are trying to inject your sample from a syringe into the injection valve.</span></div>
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<span style="font-size: small;"><br /></span></div>
<div style="text-align: left;">
<span style="font-size: small;">Anyways I'll leave it up to you guys to decide what you think of each, here some pictures!:</span></div>
<span style="font-size: small;"><o:p></o:p></span><br /></td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjfi-f4VcyvSXPFsIVobPdntjWzzwaIwfadF5jdYQX4MUfLqL8J2A_2MlOUf3oOpyL1RZuH638od6D8QLJhJ9WFulZ5SpGs-zbFN2kCyXoVXDPnxb4V5xtNaV0_vZYUzYju83mfBK223Os8/s1600/DSC04592.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjfi-f4VcyvSXPFsIVobPdntjWzzwaIwfadF5jdYQX4MUfLqL8J2A_2MlOUf3oOpyL1RZuH638od6D8QLJhJ9WFulZ5SpGs-zbFN2kCyXoVXDPnxb4V5xtNaV0_vZYUzYju83mfBK223Os8/s1600/DSC04592.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Do you see what I mean? The fractions are nicely placed in this circular spinning part of HPLC, everything is compact and nicely in placed! If you go back to my blog post about FPLC you can see the fraction collection part of the machine isn't as nicely compacted as HPLCs!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgRp_QR1eOZInXomC7AGKJuksqx7z75hUeW_VKQPOQ5lH0FfONqonnmUkxO6h2oVFEv1nYIqaipIE_jPsUmfsKhPEeTl2HLWTaDl3ndr9UJSUi7ee3yqbBdbO0fkPzIftuNNTb-I-HgTnXR/s1600/DSC04593.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgRp_QR1eOZInXomC7AGKJuksqx7z75hUeW_VKQPOQ5lH0FfONqonnmUkxO6h2oVFEv1nYIqaipIE_jPsUmfsKhPEeTl2HLWTaDl3ndr9UJSUi7ee3yqbBdbO0fkPzIftuNNTb-I-HgTnXR/s1600/DSC04593.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is Petra adjusting the HPLC machine, checking if everything is set properly. Above of the machine we have open the flap , where we can see the buffer wires. We can observe if the machine is working properly if there are no bubbles in the buffer wires and if the buffers are actually moving through the tube!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhx3FmjJx9uOPwIjvk_phuqqZ586I21oqjTc2PkTWcVd6i0nzDnhCWolGEClY8RF13DTDvgz9l5uu7b2xi55rngYWxGFynob6p7QEIvk02NqMaynrCD8zbpP-7UEHMXHZ9ufHnBmUNoerPy/s1600/DSC04594.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhx3FmjJx9uOPwIjvk_phuqqZ586I21oqjTc2PkTWcVd6i0nzDnhCWolGEClY8RF13DTDvgz9l5uu7b2xi55rngYWxGFynob6p7QEIvk02NqMaynrCD8zbpP-7UEHMXHZ9ufHnBmUNoerPy/s1600/DSC04594.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Our very concentrated A8 fraction sample of PSII. From our second run of FPLC and isolation of stacked thylakoids.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhJut7DOAFYz5khuhq3ZwDmaAYtOclVbv8oCzBk7JNw65XM2CQWeVW0lxPyWYckI2uBfgM3HaootzIr1rhKf3vWoIAhDb9tfZ5hcJ1pjBtkyZE8LRte6KWOoK3X_PWu-76mUr502pQFzSUQ/s1600/DSC04595.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhJut7DOAFYz5khuhq3ZwDmaAYtOclVbv8oCzBk7JNw65XM2CQWeVW0lxPyWYckI2uBfgM3HaootzIr1rhKf3vWoIAhDb9tfZ5hcJ1pjBtkyZE8LRte6KWOoK3X_PWu-76mUr502pQFzSUQ/s1600/DSC04595.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Labelled vials for the different isolated PSII fractions from different experiments. The number 1's are from the 1st isolation of stacked thylakoids. 1c being the most concentrated, 1b being a 1:1.25 dilution and 1a being a 1:2 dilution. The number 2's are taken from the 2nd isolation of stacked thylakoid experiment. C being the most concentrated going to slowly up to a lower dilution factor. 2 labelled vials are non acidified samples and, 3 labelled vials are acidified sample.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgPabTdNFBEfZP2SRP0eU1yHXq9FvQCK8Ou7snPvp9ehA60DzFzbIJNIVvhFfFn6HuPk8h7lDiQvekLBa126cZ-xZVZTwXjeByfujy_u18Cp_5qdi-oJscTaFlhj9m9_uShvVCUnNbWahK4/s1600/DSC04596.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgPabTdNFBEfZP2SRP0eU1yHXq9FvQCK8Ou7snPvp9ehA60DzFzbIJNIVvhFfFn6HuPk8h7lDiQvekLBa126cZ-xZVZTwXjeByfujy_u18Cp_5qdi-oJscTaFlhj9m9_uShvVCUnNbWahK4/s1600/DSC04596.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">All the vials nicely placed in, if you see to the back of the machine there is basically a needle attached to the metal moving thing. This is the needle which injects into the vial and samples for the HPLC run.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicP1UhPcD6Kehp1TbSnZcRJpjLrpovHCwigf6-7Qfb8H2QSvGf63gfFkmHRt0j-LNAAoQoqV9tCBW-f4EaRURlnT3JMqXc6y11bffQdPaO2GT1_kI68aAi5jdjo7YcbsqSKvsDihsMqlXN/s1600/DSC04597.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicP1UhPcD6Kehp1TbSnZcRJpjLrpovHCwigf6-7Qfb8H2QSvGf63gfFkmHRt0j-LNAAoQoqV9tCBW-f4EaRURlnT3JMqXc6y11bffQdPaO2GT1_kI68aAi5jdjo7YcbsqSKvsDihsMqlXN/s1600/DSC04597.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Side view of HPLC</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi2oo2usEhi9oJQ0HkV_iETAJqTZ_AXMWeIfDexjB2jkK8LcCb9_LLyqnnTn-1w7LFpV-_Mo80HIKG7pVGziMoArWK_wd1wJxfO_PvzUfNZUafrGxzn4iKFjzLrlOMrEJrvRCj6ZPboLYDQ/s1600/DSC04599.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi2oo2usEhi9oJQ0HkV_iETAJqTZ_AXMWeIfDexjB2jkK8LcCb9_LLyqnnTn-1w7LFpV-_Mo80HIKG7pVGziMoArWK_wd1wJxfO_PvzUfNZUafrGxzn4iKFjzLrlOMrEJrvRCj6ZPboLYDQ/s1600/DSC04599.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">A better picture of all the vials in place with their caps on. In the middle of the cap is a circular paper disc which the needle can easily pierce.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjNZC8St-WQsGWazhLWku7_kFLpZojymilehMNHtiPM6ghTysEtZFlit7SyCS0-4zUxxl8NCE-Iilwph_XbpD47tRXfwlc0nzFcFw_6qKogv6Xoq2p2ndQmGDZARUhpN4_dElP6TkCua_Qx/s1600/DSC04600.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjNZC8St-WQsGWazhLWku7_kFLpZojymilehMNHtiPM6ghTysEtZFlit7SyCS0-4zUxxl8NCE-Iilwph_XbpD47tRXfwlc0nzFcFw_6qKogv6Xoq2p2ndQmGDZARUhpN4_dElP6TkCua_Qx/s1600/DSC04600.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Computer part of the HPLC. Yes you can clearly see windows 2000! You might be thinking why is this not windows 8?! Or something more up to date? Basically most of these chromotography or any lab machines were built a while back and obviously configured to work with windows 2000 only. For Ruban's lab to change and update everything to windows 8 could take a process of an entire month. So if it works then its fine. Despite the fact it is awfully slow!!!! -.-</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhs7yI-nfjccFtk0SqlwtE6SzxRE6mwxD0ZMtYDnfp18kCUxJVWDimz_gNRnLrBEAQlXBX6i65VariVz_Bu-H3IIkVm3OrwynQ15APLGtDgBw9Ii4R1ngk_GbhgHh3UxgjTf70XEK81KkYA/s1600/DSC04601.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhs7yI-nfjccFtk0SqlwtE6SzxRE6mwxD0ZMtYDnfp18kCUxJVWDimz_gNRnLrBEAQlXBX6i65VariVz_Bu-H3IIkVm3OrwynQ15APLGtDgBw9Ii4R1ngk_GbhgHh3UxgjTf70XEK81KkYA/s1600/DSC04601.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">With the lid on!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjLVaXkewE4MY6umX120fQbig-65kwOu9yNg3JkZbUWzd1_x1wEOmheRP2kpS6vWkEERzrO26lYuSSFsApe7zg_9Hi5nBkjEFa8TTdY0-lLbLrB85pEqMXmLz4CgsFGxSfahQRP_DXGtRx8/s1600/DSC04602.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjLVaXkewE4MY6umX120fQbig-65kwOu9yNg3JkZbUWzd1_x1wEOmheRP2kpS6vWkEERzrO26lYuSSFsApe7zg_9Hi5nBkjEFa8TTdY0-lLbLrB85pEqMXmLz4CgsFGxSfahQRP_DXGtRx8/s1600/DSC04602.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Lovely windows 2000 again! Did i mention it was windows 2000 professional?! :O</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhJg3p47DkRbOQu7jXBsLYBAFNUlMKviZ2Ls3AbJ605rQd6o-HQrsE6gd0b1_hMEmiRFJxadhpIpZXyppAtyZWvEZnrhCFNxaImiErULvQVszCn9htFIu3V-jqFSASmhqmPr907B3G7t4nD/s1600/DSC04604.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhJg3p47DkRbOQu7jXBsLYBAFNUlMKviZ2Ls3AbJ605rQd6o-HQrsE6gd0b1_hMEmiRFJxadhpIpZXyppAtyZWvEZnrhCFNxaImiErULvQVszCn9htFIu3V-jqFSASmhqmPr907B3G7t4nD/s1600/DSC04604.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Liquid nitrogen, being daring and placing my hand on the vapor ! Basically its fine as when the vapour touches your skin it immediately evaporates! Don't ever actually immerse your entire hand in the liquid nitrogen its equivalent to putting your hand in burning oil, besides your hand would freeze in pain. For me I think freezing to pain is worst as I am so sensitive to cold. I remember doing a lab experiment to see how tolerant I was to placing my hand in icy waters, yeah I didn't last long....*roll eyes* :)</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiV6x9TU-ZYWUp3-1zpHWns3htXzmXcfy1DZHPkdOREuZeMImP__VuhaW_opPpPxeDijB5ikoFtMlhwnJIwqsBIhV-MGT0d4qZ8bXTkL84lKbXVk2WQhpyA3o1YjqjyigeNKRW5ZKnIxHH6/s1600/DSC04605.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiV6x9TU-ZYWUp3-1zpHWns3htXzmXcfy1DZHPkdOREuZeMImP__VuhaW_opPpPxeDijB5ikoFtMlhwnJIwqsBIhV-MGT0d4qZ8bXTkL84lKbXVk2WQhpyA3o1YjqjyigeNKRW5ZKnIxHH6/s1600/DSC04605.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The program that processes the data collected and analysed by HPLC. A nice mouse mat which probably came with the software! How kind of Chromeleon!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgNcw1XK79tzqnNofbIT6L6CCjNtzpqXj_gc0dHFWTASTSLDGUByQN3j-s7HINWu7O3Es5C2K13prcYOVzbMOchjD7QyITEvmWwUAs7xpPeaxY8M93rPe5M_LpI9Z0B5HhuVuYVoX9tRY84/s1600/DSC04606.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgNcw1XK79tzqnNofbIT6L6CCjNtzpqXj_gc0dHFWTASTSLDGUByQN3j-s7HINWu7O3Es5C2K13prcYOVzbMOchjD7QyITEvmWwUAs7xpPeaxY8M93rPe5M_LpI9Z0B5HhuVuYVoX9tRY84/s1600/DSC04606.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Chromeleon on the desktop loading!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgtyITRycRFCxVOBykeGUna_rhWjZqEVtFBaCLFkSyHGIK-KPZQD516J5lyrSKacrOqulCuwrvdwNgclDFTivgFPhjmaQ_msi2UBCpMGKfSSocl0iNEWtkyZAd7p5qEcCXJPiXHUjXdb_HQ/s1600/DSC04607.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgtyITRycRFCxVOBykeGUna_rhWjZqEVtFBaCLFkSyHGIK-KPZQD516J5lyrSKacrOqulCuwrvdwNgclDFTivgFPhjmaQ_msi2UBCpMGKfSSocl0iNEWtkyZAd7p5qEcCXJPiXHUjXdb_HQ/s1600/DSC04607.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Literally, this is five minutes after and yet it still fails to load.... well it did load eventually but obviously windows 2000 is old, it's gonna be slow!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhFSKIHCFsn4wvyaHUwfTLj7ltv9NkZxUu18Ke5fYncA_iofdSlEua_1ZZ9uabbuGSeUjjYjwtGWXeMqoSZvSKc8mEXUlzORad8-shdjvmKc6_CqCrLp-dbGRt-50a_rApqhhz4y6TFPNgC/s1600/DSC04608.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhFSKIHCFsn4wvyaHUwfTLj7ltv9NkZxUu18Ke5fYncA_iofdSlEua_1ZZ9uabbuGSeUjjYjwtGWXeMqoSZvSKc8mEXUlzORad8-shdjvmKc6_CqCrLp-dbGRt-50a_rApqhhz4y6TFPNgC/s1600/DSC04608.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Setting the parameters for our HPLC run~ oooohhh colours!!!! You might wonder how on earth would I know how to use this as a undergrad, wouldn't I get confused? Basically we have this HPLC for dummies walk through which I just follow the instructions to~ it is slightly overwhelming, but if you can read you'll be fine :P<br />
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To conclude, this was a very interesting day. The HPLC run was basically a test run as our fraction samples did not have 100% PSII complexes only, they were not fully unsolublised. Today was basically for me to see how HPLC works and to get use to the machine itself :)<br />
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J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-20415246620154165142014-06-04T21:30:00.000-07:002014-06-11T10:57:42.033-07:00Second repeat of the stacked thylakoid preparation <div class="separator" style="clear: both; text-align: left;">
Having already talked about the basis of this experiment in the previous blog. I decided just to take pictures of this time repeat, so show you more about the lab :)</div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhkOdspTE6SYXfG9K24aClp7gOnrpJG7cejKbrFv2_H4J7kCLN_bV0_2t2HGeQA_Et_hAv0SdQou4_9rvwUYKRQhqg-ddCjhLfgpbTwyOKMqNw-u-ZEaqywvLjkJVLYKbRduVhF-KPnHy_C/s1600/DSC04386.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhkOdspTE6SYXfG9K24aClp7gOnrpJG7cejKbrFv2_H4J7kCLN_bV0_2t2HGeQA_Et_hAv0SdQou4_9rvwUYKRQhqg-ddCjhLfgpbTwyOKMqNw-u-ZEaqywvLjkJVLYKbRduVhF-KPnHy_C/s1600/DSC04386.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="font-size: small;">Again and FPLC machine~</span></td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjYXO-IGVmv4RwsJcQwXX80bv54Dce6v3hMowaNVrz4amf2fyHmqAs7CQ_3mUxW-YRFjBgnBPARSb66NJBkMY1aGHKSyFwJLFRm-SncnTq3V4lOzYDbxjTHcfPTiFSPD9AzB980qm0GPtTH/s1600/DSC04387.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjYXO-IGVmv4RwsJcQwXX80bv54Dce6v3hMowaNVrz4amf2fyHmqAs7CQ_3mUxW-YRFjBgnBPARSb66NJBkMY1aGHKSyFwJLFRm-SncnTq3V4lOzYDbxjTHcfPTiFSPD9AzB980qm0GPtTH/s1600/DSC04387.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="font-size: small;">The FPLC & HPLC machines next to each other. HPLC machine is the white grey machine next to the FPLC machine. The FPLC includes the yellow machine next to it with many test tubes placed in it.</span></td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiebUtVOaa3rPGKBKy4CdCHttNu83VUJ0rklOyhv2dpNiEPNpXlmP_kyKZThJIn50G4-PSjXSUKd7G22lV4oW0D3UMQRANsvt8jNORiXMl8iNAMs-wbks75FUf1OhZjr0UBca9-1I7xGx8K/s1600/DSC04388.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiebUtVOaa3rPGKBKy4CdCHttNu83VUJ0rklOyhv2dpNiEPNpXlmP_kyKZThJIn50G4-PSjXSUKd7G22lV4oW0D3UMQRANsvt8jNORiXMl8iNAMs-wbks75FUf1OhZjr0UBca9-1I7xGx8K/s1600/DSC04388.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">General notes~</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh4EYs970khjMvYQQcv7YbBI4oXYFYGLMJMxxFU3xPI17w7OjG5pIBMkNKu91iXOi1nNn38qZ0c5w0dOXPgM6SHr9FWvZpnT5KhdxpnKDY0ez-Py2Iw97-Kv10b8H5cBOJRMpqHx06fmSZd/s1600/DSC04389.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh4EYs970khjMvYQQcv7YbBI4oXYFYGLMJMxxFU3xPI17w7OjG5pIBMkNKu91iXOi1nNn38qZ0c5w0dOXPgM6SHr9FWvZpnT5KhdxpnKDY0ez-Py2Iw97-Kv10b8H5cBOJRMpqHx06fmSZd/s1600/DSC04389.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is a magnetic hotplate stirrer. You basically place an metal stirrer at the bottom of the beaker and adjust the right knob for the speed of spinning the metal stirrer.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiadAZCH2E_EVRvOj7eSYPeuNtrFB8Rrpi9A08QOvmgjwvYl3PoIgGi3D8TqDITB6ZmpWVuPPOYjgovw-oXv2LCS1IH-m4SYioUi7pSW81x6C0CMmavTXe3XCkhWkDGYDVSY4yUgucno9ZT/s1600/DSC04390.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiadAZCH2E_EVRvOj7eSYPeuNtrFB8Rrpi9A08QOvmgjwvYl3PoIgGi3D8TqDITB6ZmpWVuPPOYjgovw-oXv2LCS1IH-m4SYioUi7pSW81x6C0CMmavTXe3XCkhWkDGYDVSY4yUgucno9ZT/s1600/DSC04390.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is a pH meter. the probe stuck into a test tube covered in film is the actual meter which you place in a solution.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgyxn7ISOOMZihUrkkp1zBexz4BDO-VJxixXFFcNYdHrMkpwhssikUPyP8P4IJG5Qp5oX_TaK3dqeriKgSGzcYtlFG45udQ_P6i6podPr07ip6JeFyQDabIyYPhrtNHvkMz3tWOQc6AV0lb/s1600/DSC04391.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgyxn7ISOOMZihUrkkp1zBexz4BDO-VJxixXFFcNYdHrMkpwhssikUPyP8P4IJG5Qp5oX_TaK3dqeriKgSGzcYtlFG45udQ_P6i6podPr07ip6JeFyQDabIyYPhrtNHvkMz3tWOQc6AV0lb/s1600/DSC04391.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Super sensitive weighing scales, measuring to 4dp! It has doors on the top and both sides. You basically open both sliding doors on both sides and use one hand to spatula in the fine material into the dish and the other hand holding the tub of material.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjw_sXRo2vHJK3zuCZpEvH5oXnY6JBVma_iGVlWSSVdJfaAj-SOaPTD-YpRMeU0xPQCxHteU-dMvOjQufvfcaiOMM5cgFBH1v1nc2Xy8BHbYUH6siGOO860vowmkjd-OEexMMtKSgzCIrnO/s1600/DSC04392.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjw_sXRo2vHJK3zuCZpEvH5oXnY6JBVma_iGVlWSSVdJfaAj-SOaPTD-YpRMeU0xPQCxHteU-dMvOjQufvfcaiOMM5cgFBH1v1nc2Xy8BHbYUH6siGOO860vowmkjd-OEexMMtKSgzCIrnO/s1600/DSC04392.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">All the chemicals!!!</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjtmt2Sv_8wF7JkPFvIdab7_N0c7GDPHJQiKfQr4wB5jyhq8rwfz7waOPAaWM_V3cwnW8tGUadMFOCu6q_AHyVYGruzD1dmYLgQntXE2b7_3QvYUl-H_VfEZQ0CGKMI4mHSPhjk_7uLuhvq/s1600/DSC04393.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjtmt2Sv_8wF7JkPFvIdab7_N0c7GDPHJQiKfQr4wB5jyhq8rwfz7waOPAaWM_V3cwnW8tGUadMFOCu6q_AHyVYGruzD1dmYLgQntXE2b7_3QvYUl-H_VfEZQ0CGKMI4mHSPhjk_7uLuhvq/s1600/DSC04393.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Really cool pipette and futuristic looking. Used one for the first time today for pipette larger volumes of up to 10ml. Really cool you have to get use to the different types of pressure applied to the button.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgwyxaQ5hsEkHaEIz_WdKFk-FUrFcI8Lehyphenhyphen6cL-TdEGPgCqe4_eIDe18Yk642-F7xmOkW3MtPlhnCqzKQM_Xl5U6Feh1x9A1sqJs6FnXMtcBziSfCGgUSscZZPrNUylYs4HRFAhaidcfgIs/s1600/DSC04394.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgwyxaQ5hsEkHaEIz_WdKFk-FUrFcI8Lehyphenhyphen6cL-TdEGPgCqe4_eIDe18Yk642-F7xmOkW3MtPlhnCqzKQM_Xl5U6Feh1x9A1sqJs6FnXMtcBziSfCGgUSscZZPrNUylYs4HRFAhaidcfgIs/s1600/DSC04394.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">A pathway of the FPLC machine~ where the injected material travels along.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgJRIEqBc1EkUPpNCp4eKlDNOPKCuHUd_TawmGr3h7J6pIDMfzhL5ouY83-Jk6l42UbFcQTjmlP7FDYZASkqWRl6m_1Vj73LjOmrvwT2IhYVQCkj_jYRVFLnLYBLZ_H5uWMFqzxeRJW-VVJ/s1600/DSC04395.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgJRIEqBc1EkUPpNCp4eKlDNOPKCuHUd_TawmGr3h7J6pIDMfzhL5ouY83-Jk6l42UbFcQTjmlP7FDYZASkqWRl6m_1Vj73LjOmrvwT2IhYVQCkj_jYRVFLnLYBLZ_H5uWMFqzxeRJW-VVJ/s1600/DSC04395.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Pump A of the FPLC!</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhVnkSWEHQGLPWc5un3TJ0lnk_88XGigQ6b_1KB54kvBSiwM51iNGMp3u5xzUHdhGncPhXVTEK2IBcKqgaOrQyN2lFk440B4iW-tNrRQHIW9gYiL-k50WKLSf9PNyE-rcamBYawrn5Zajm7/s1600/DSC04396.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhVnkSWEHQGLPWc5un3TJ0lnk_88XGigQ6b_1KB54kvBSiwM51iNGMp3u5xzUHdhGncPhXVTEK2IBcKqgaOrQyN2lFk440B4iW-tNrRQHIW9gYiL-k50WKLSf9PNyE-rcamBYawrn5Zajm7/s1600/DSC04396.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The injection valve of the FPLC, we use a syringe to inject our sample into this.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiuhzQyimajYJlN_C9R55mud84Hu1kVrmuAvvTj88EZhKbikAXn8fN-IB8wFLE56l-BtEKz2S-90s4DPFmNYJDoqkJHtXXe4_fMLmJQKbIlc3OyOx6EqFO2R2n2nDM4ZfsKgLh8XUQY4UZv/s1600/DSC04397.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiuhzQyimajYJlN_C9R55mud84Hu1kVrmuAvvTj88EZhKbikAXn8fN-IB8wFLE56l-BtEKz2S-90s4DPFmNYJDoqkJHtXXe4_fMLmJQKbIlc3OyOx6EqFO2R2n2nDM4ZfsKgLh8XUQY4UZv/s1600/DSC04397.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The picture showing the different loops connecting to different compartments in the FPLC.</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgrktg0JKtdEnuHMGcldMgV_BBv6f7PiNg3uT96fvqMwWuf9TgBVyFcCgfJCWSPA56VjHXp3f68fjkJj-pf6yqN6Dul1D73N90l4ORtOTNH6RN7UeVOsOsCKNTShXmtmP5jpJBcWLPe_G2z/s1600/DSC04398.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgrktg0JKtdEnuHMGcldMgV_BBv6f7PiNg3uT96fvqMwWuf9TgBVyFcCgfJCWSPA56VjHXp3f68fjkJj-pf6yqN6Dul1D73N90l4ORtOTNH6RN7UeVOsOsCKNTShXmtmP5jpJBcWLPe_G2z/s1600/DSC04398.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Clearly, this is where the fractions from FPLC are collected...they are labelled in series as A1 A2 A3, B1, B2, B3...etc</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjFQVQwi1Cu8mtlizKioyr5jXot-sRiMObn5WQx5SQPZ59B0t2lMj4VzM_aQas9gKV95yhrYoaEZ3Cd9Adan3GfLvunoH7-JZ9G-k7MWzzTy5TNx6axAgn-00HmbC_5GEZsOjbjyDDQ34aq/s1600/DSC04399.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjFQVQwi1Cu8mtlizKioyr5jXot-sRiMObn5WQx5SQPZ59B0t2lMj4VzM_aQas9gKV95yhrYoaEZ3Cd9Adan3GfLvunoH7-JZ9G-k7MWzzTy5TNx6axAgn-00HmbC_5GEZsOjbjyDDQ34aq/s1600/DSC04399.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">How we use the programme to set the parameters desired.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj8hmgYaUC7xP5AwxxcQqcQtGnjdViEI9ulqMjM84uMihoVzmKTvwiPbv7hgqP-sObYtw2B_qDLYg8bMMDFvsjwA2Mheq-QeH2E6zoD5xAi1AzfpBjnz1VEtuzkCy9xbNK5envf3LaImBxn/s1600/DSC04400.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj8hmgYaUC7xP5AwxxcQqcQtGnjdViEI9ulqMjM84uMihoVzmKTvwiPbv7hgqP-sObYtw2B_qDLYg8bMMDFvsjwA2Mheq-QeH2E6zoD5xAi1AzfpBjnz1VEtuzkCy9xbNK5envf3LaImBxn/s1600/DSC04400.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Pump A again!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgL1_pQRUWZL-1XEPFit8HIwbsOlp3c2RQAL_dApKSIQRyugHsFhUj8CQQCf4Iu3zQhFphSdPjPVuvqF605-_ny22wc9PCgAz1BfLkI_k3ujRxFQPs2kJWZEaNw0ySLWokftn-KGu78fc7A/s1600/DSC04403.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgL1_pQRUWZL-1XEPFit8HIwbsOlp3c2RQAL_dApKSIQRyugHsFhUj8CQQCf4Iu3zQhFphSdPjPVuvqF605-_ny22wc9PCgAz1BfLkI_k3ujRxFQPs2kJWZEaNw0ySLWokftn-KGu78fc7A/s1600/DSC04403.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">My work desk in the lab!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-HzD48Y7tPvKbMvPZ4FI_DIkg0fiO58FuKhi_VoLSrHtYUl7UhzvW-tjICWMg9ySFwK04VQufoISgvKEbAlmRDwzsEgdl4lkHZjCP6aPnpfMTajvbvUvU5h4-aEQaVGXVYNfX5ycA_9zq/s1600/DSC04404.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj-HzD48Y7tPvKbMvPZ4FI_DIkg0fiO58FuKhi_VoLSrHtYUl7UhzvW-tjICWMg9ySFwK04VQufoISgvKEbAlmRDwzsEgdl4lkHZjCP6aPnpfMTajvbvUvU5h4-aEQaVGXVYNfX5ycA_9zq/s1600/DSC04404.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The superdex column, this time extremely green, probably due to the fact we increase our sample loop volume to 500ul!</td></tr>
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<br />J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-35900114472398946702014-06-03T18:00:00.000-07:002014-06-08T08:44:54.308-07:00My beautiful lab book!<u>Today:</u> Day 3 was basically preparing buffer solutions for our next run of repeating the experiment and using FPLC again! It was basically a two hour lab session, I asked many questions too~took many notes.<br />
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Anyways, today blog is about my beautiful lab book!~ Generally, I am an individual who likes organisation and neatness in life :)<br />
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When I first started this project, I was basically using an ordinary pukka pad, jotting very messy notes onto the pad. However, when I went back home I found that it was really hard to understand my scrawny notes and the organisation of the pages/ additional print out pages very messy. In general mess for me leads to stress!<br />
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So I went to my University stationary shop near the labs and brought a suitable lab book. I had chosen this one because it was mostly plain but I liked the stripes of colour down the main page. Also, as discussed with Pedra, lab books aren't meant to have pages which you can easily rip out.<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjEupaSk29xKERtxllWeGYPatg2nVJQ8Jjyv3_juCjryPJwj5-0Ou5ihFvnZcwEsP1IORHGsBYWVqXSjRECvX08rNW6DcM5tb1gLui3KPQvzYh2R3OnSDPWD9QJ2yR_9uxuQOI9TFmivPxH/s1600/IMG_20140608_155734.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjEupaSk29xKERtxllWeGYPatg2nVJQ8Jjyv3_juCjryPJwj5-0Ou5ihFvnZcwEsP1IORHGsBYWVqXSjRECvX08rNW6DcM5tb1gLui3KPQvzYh2R3OnSDPWD9QJ2yR_9uxuQOI9TFmivPxH/s1600/IMG_20140608_155734.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">My beautiful lab book! See what I mean it seems plain but the streaks of colours really attracted me towards this notebook! :) On the left, its Ruban's written photosynthetic membrane book! Really good read if you are in this field. He was written it in such a way that it is easy to understand, compared to alot of textbooks out there I have read. Also it is very concise, gets right to the point!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi_SOSLa6kj7DpLcdoFBrJK8SK0YHH-DuvUSHWMTSGWUqUCRY1fyuvbnrii8bV93e3paJv3eL9wd3Dy_qvJTGEQxj6Kqz1-fkoLCMqwHIOjdmK212Frk4rY6ZHVJTbFs4bhzd_pTnEagBF6/s1600/IMG_20140608_155740.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi_SOSLa6kj7DpLcdoFBrJK8SK0YHH-DuvUSHWMTSGWUqUCRY1fyuvbnrii8bV93e3paJv3eL9wd3Dy_qvJTGEQxj6Kqz1-fkoLCMqwHIOjdmK212Frk4rY6ZHVJTbFs4bhzd_pTnEagBF6/s1600/IMG_20140608_155740.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The importance of having an index page. Reminded from Pedra! Helps you easily locate experiments done! Organisation is the way forward!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicgFf-FoLyXPO7tKaLcZ85TNLQxKyGJ-HLpVXbMaT4nuzss_Z9JFGW9BDLEsYD4858vBgVDwAVc-eBsZJoQCdgVMVoozyeCZUewWuhTwmUbJSRdBfbx8Cgs47O0Febpg7Wq5YrnNnOu2Dh/s1600/IMG_20140608_155749.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEicgFf-FoLyXPO7tKaLcZ85TNLQxKyGJ-HLpVXbMaT4nuzss_Z9JFGW9BDLEsYD4858vBgVDwAVc-eBsZJoQCdgVMVoozyeCZUewWuhTwmUbJSRdBfbx8Cgs47O0Febpg7Wq5YrnNnOu2Dh/s1600/IMG_20140608_155749.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">So the outline for the 8 week studentship! Now I understand the general experiment just repeats, repeats, repeats with different variables! </td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg0Irys4w2wuaaXDEvXgEOP5BYkb8NsCSwjRVvi-BDUzdnKrlEOl6HaFveTcUQo53dJnulSyjadAReUGWLewYkzN43qOdus6Q6BK3SFarlIgv3wC4jVNZsztNJraYCOX_EYof_7_QBYlLFh/s1600/IMG_20140608_155802.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg0Irys4w2wuaaXDEvXgEOP5BYkb8NsCSwjRVvi-BDUzdnKrlEOl6HaFveTcUQo53dJnulSyjadAReUGWLewYkzN43qOdus6Q6BK3SFarlIgv3wC4jVNZsztNJraYCOX_EYof_7_QBYlLFh/s1600/IMG_20140608_155802.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">These are the instructions that I have made into booklet size so I can stick them into my lab book neatly. I really didn't like the A4 printout stable version of them before, they were messy to deal with, now that they are in booklet size, they fit nicely in my lab notes ^_^</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEitiK9gkZIYEwPsnmmoVlQeFmWS2Eo30azwHaH1yV616vEG2koByR4i21TQNGAr4r_oK5S-GqftoF_razkYNylZ06LSoDTy-wiPNRJ2NDy4Ta7SQaexYKSZXTf15LapimHtVq37Tce6j2B0/s1600/IMG_20140608_155810.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEitiK9gkZIYEwPsnmmoVlQeFmWS2Eo30azwHaH1yV616vEG2koByR4i21TQNGAr4r_oK5S-GqftoF_razkYNylZ06LSoDTy-wiPNRJ2NDy4Ta7SQaexYKSZXTf15LapimHtVq37Tce6j2B0/s1600/IMG_20140608_155810.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Preview of my lab book, basically i really try writing in a neat manner when in labs! I just don't have the time to write it messy and rapidly first time, then copy them up after lab. I actually have a life outside lab too :P</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgIdPUE9r3Na6Jt_0K-dUdQHaL132sPVkRXkf5CdgmGKGEqGbJHf6WbTbEw33BgAKszzM5ppNC8uOuRI0TaJoDHp3jX8c1-QzAwrcww_I4AzH1rh1iAfeVfSdo51gdjr6dhgp65C4racx-N/s1600/IMG_20140608_155817.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgIdPUE9r3Na6Jt_0K-dUdQHaL132sPVkRXkf5CdgmGKGEqGbJHf6WbTbEw33BgAKszzM5ppNC8uOuRI0TaJoDHp3jX8c1-QzAwrcww_I4AzH1rh1iAfeVfSdo51gdjr6dhgp65C4racx-N/s1600/IMG_20140608_155817.jpg" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">These were rough pencil notes made about the equipment and experimental steps during day 2 when analysing results, I basically asked follow up questions to do with things that I wasn't sure about in the experiment!</td></tr>
</tbody></table>
To conclude! The neater and more organised your lab book is, the nicer it is to look back at it during repeating experiments step! As you can tell from the picture, because I have put so much time and effort into my lab book, it really is such a nice piece of art to visually look at/ read :)J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-61637513482101231012014-05-30T17:30:00.000-07:002014-06-11T10:54:37.559-07:00Analysis of results, from day 1 experiments<div class="Publishwithline">
So this Friday we basically just went in and analysed our
results from FPLC the other day, having not had time the other day~</div>
<div class="MsoNormal">
<br />
So we found a mysterious curve at the end of our results~ which indicates that
there a lot of protein in our final sample where we have solublised the
photosynthetic membrane.<o:p></o:p></div>
<div class="MsoNormal">
Despite that~ we have quite a large amount of photosystem II
(PSII, which I will now refer to), which is the fraction we really need for the
next step of analysing on HPLC.<br />
<br />
We came to the conclusion, that next time we want less free pigment trimmers,
indicated on the graph before, as we want more of the PSII.<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg3l_Z3XFbDZgq7ubPaMAVygegCnRXI3R4AoQ9sYWN7JvFAKub06cgJfjkmgu3EQ0ey5xuArzKQU_qPUrArgu8jG8UOGp-yLvCpsirZs-5wGqbT4SZQpxACakdftBuZs1nwlejD4ltFX_XB/s1600/IMG_20140608_155719.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg3l_Z3XFbDZgq7ubPaMAVygegCnRXI3R4AoQ9sYWN7JvFAKub06cgJfjkmgu3EQ0ey5xuArzKQU_qPUrArgu8jG8UOGp-yLvCpsirZs-5wGqbT4SZQpxACakdftBuZs1nwlejD4ltFX_XB/s1600/IMG_20140608_155719.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The graph produced via FPLC. My analysis notes in my lab book! I'll talk about my lab book in another blog :)</td></tr>
</tbody></table>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<u><b>Therefore next time when we repeat the experiment: we will
use</b></u><br />
<u><b><br /></b></u>
<span style="font-family: Symbol; text-indent: -18pt;">·<span style="font-family: 'Times New Roman'; font-size: 7pt;">
</span></span><span style="text-indent: -18pt;">A greater sample loop for injecting into the
sample loop. This is because our fractions came out extremely pale and didn’t
have much green at all. If we concentrate our fractions by having a greater
volume, it means that next time our fractions will be a greener colour. We increased
the sample loop of the FLPC from 100ul to 500ul. However, the use of 500ul
might result in a poorer resolution in our results as 500ul is the maximum
volume that the sample loop can hold.</span><br />
<span style="text-indent: -18pt;"><br /></span>
<span style="font-family: Symbol; text-indent: -18pt;">·<span style="font-family: 'Times New Roman'; font-size: 7pt;">
</span></span><span style="text-indent: -18pt;">There is a chance that our buffer could have
over solubilised our supercomplexes, giving us more free pigments than
expected. Hence next time we repeat this experiment, we can try changing the
concentration of the buffet- alphaDM to 0.8% instead of using 1%. This will
hopefully give us a larger amount of PSII complexes obtained in the fractions
next time </span><span style="font-family: Wingdings; text-indent: -18pt;">J</span></div>
<div class="MsoListParagraphCxSpLast" style="mso-list: l0 level1 lfo1; text-indent: -18.0pt;">
<o:p></o:p></div>
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<span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;"><span style="mso-char-type: symbol; mso-symbol-font-family: Wingdings;"><br /></span></span></div>
<w:sdt contentlocked="t" id="89512093" sdtgroup="t"><span style="font-family: "Calibri","sans-serif"; font-size: 1.0pt; mso-ansi-language: EN-GB; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: "Times New Roman"; mso-fareast-language: EN-GB; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin;"><w:sdtpr></w:sdtpr><w:sdt docpart="C1F99C7F16774DE6AC5CF7CDB6942CD8" id="89512082" storeitemid="X_5F329CAD-B019-4FA6-9FEF-74898909AD20" text="t" title="Post Title" xpath="/ns0:BlogPostInfo/ns0:PostTitle"></w:sdt></span>
</w:sdt>
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<div class="MsoNormal">
I find the analysis of results the best part of experiment.
I enjoy seeing outcome after a long day of slaving in the lab. I can’t wait to
repeat this experiment and see what changing one variable will have an effect
on the results! Also, the next time I do this experiment I will be overall more
confident and know what each step is~ hence be more efficient in the lab!</div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
Due to the fact my summer project is supervised with the lab's technician, Petra, my entire timetabling depends around her timetable. For example I do not need to come in next monday, instead I can do extra reading. This means that I'm not in everyday at the lab~ some days I actually have off to do summer related things, like going out to see friends :D Which just makes me feel even more luckier to be awarded with such studentship, as the project itself is independent- its a project of my own and not a project or another phd student. Also, its a nice summer studentship as its not 9-5pm everyday, giving me the time to actually enjoy summer! Win Win Win!<br />
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J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-36450246765273981492014-05-29T21:00:00.000-07:002014-06-11T10:55:23.678-07:00First day in labs!<div class="MsoNormal">
Oh the excitement, I actually didn’t know what to expect! I’ve
been in labs before obviously, but this was a project of my own now! Where I
would be the only student doing this and basically discovering new results for
my supervisor!<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
So starting at 9am and ending at 6pm, I can honestly say it
was a long day! Obviously I had a lunch break and everything, but still very
long!<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
Thing is with these types of experiments, there is a lot of centrifuging
and treatment that needs to be done in order to get the final, correct medium
that can be finally analysed via the chromatography machine- FPLC: fast protein
liquid chromatography. In our experiment we are basically only wanted to
separate the different supercomplexes in the photosynthetic membrane; to mainly
get the fraction of photosystem II, so we can further analyse the pigments in
the photosystem II via HPLC: high performance protein liquid chromatography
that are involved in light stress. Obviously, understanding the underlying
mechanisms of the photosystem II will really aid in my future plans of further
being a researcher in artificial photosynthesis <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;">J</span><o:p></o:p></div>
<br />
<div class="MsoNormal">
<br /></div>
<div class="MsoNormal">
<u>Anyways here is photos to explain the day:</u></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjt09daJwTFTfqbZmLPURo_37DI_NzjXk32B8PzvJEfdHJRFeSUhSJgFNlEY2wv4xiJ1nGD3gznZbkwLlKaYTLO5UwvikoxkQBkONlNTCiD6307GXb83ctsgnf9IhkT-mpbRCd-kv2h9GCH/s1600/DSC04007.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjt09daJwTFTfqbZmLPURo_37DI_NzjXk32B8PzvJEfdHJRFeSUhSJgFNlEY2wv4xiJ1nGD3gznZbkwLlKaYTLO5UwvikoxkQBkONlNTCiD6307GXb83ctsgnf9IhkT-mpbRCd-kv2h9GCH/s1600/DSC04007.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">This is a device which basically spins the spinach around in hand controlled speeds, hence drying washed spinach rapidly! We obtained the spinach fresh from Whitechapel market in the morning.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgB8JXE_ZjKQaSMbZHdFq-wfRPkq7JYVljabCHMjlhwM_NhjCzahCZuLE9mQ0Z0TCBeE3C9lb-1tHi6Xc5waS5FBfnOOvlMUfxTnNLg3PgMnFXSyPaeAux4B8Som3a7i0DS_cHYmizE5NYW/s1600/DSC04009.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgB8JXE_ZjKQaSMbZHdFq-wfRPkq7JYVljabCHMjlhwM_NhjCzahCZuLE9mQ0Z0TCBeE3C9lb-1tHi6Xc5waS5FBfnOOvlMUfxTnNLg3PgMnFXSyPaeAux4B8Som3a7i0DS_cHYmizE5NYW/s1600/DSC04009.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The nitrogen tank! Looks so scary :3 It actually looks like a nuclear bomb or something in real life.... stuff which i just generally think should have never been created in this world; we should all try to live in harmony, but sadly this is not the case in our world right now :( Anyways, the technician showing me how to switch on the supply/ off.</td></tr>
</tbody></table>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgcfo88W1BwF_uTezZ5liFM33mavbB7PxYm9EW5unrVQoJvx5ndLvggIpnigZBdJqlUn1tpxUFyZ2M5_gjPE0WtQH4v_u80np2GS9_F63P6lyQRVYt8DKvrJAYVlxaQI3Geff6YSc8DwSq4/s1600/DSC04008.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgcfo88W1BwF_uTezZ5liFM33mavbB7PxYm9EW5unrVQoJvx5ndLvggIpnigZBdJqlUn1tpxUFyZ2M5_gjPE0WtQH4v_u80np2GS9_F63P6lyQRVYt8DKvrJAYVlxaQI3Geff6YSc8DwSq4/s1600/DSC04008.JPG" height="320" width="229" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="background-color: white; color: #545454; font-family: 'Open Sans'; font-size: 11px; line-height: 15.523199081420898px;">This is me chopping up the stems and midrib of the spinach preparing them for light treatment. I was initially quite slow as I didn't want to damage the spinach leaf and lose good material.</span></td></tr>
</tbody></table>
<div class="separator" style="clear: both; text-align: center;">
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiWEh3cekaxguawYLqTzFdNBcDNR9_ct7Jjaj8HvfbdapwEWh6Jkk-XaZ2oEHsb-0W-vM4O4WoW4vPFCr9rJ2FFqZXfv76SBPJrZzaACG6i7LxFbnZyh2ypWC0ns9FGpbQ0SdE6FW08f4F8/s1600/DSC04010.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiWEh3cekaxguawYLqTzFdNBcDNR9_ct7Jjaj8HvfbdapwEWh6Jkk-XaZ2oEHsb-0W-vM4O4WoW4vPFCr9rJ2FFqZXfv76SBPJrZzaACG6i7LxFbnZyh2ypWC0ns9FGpbQ0SdE6FW08f4F8/s1600/DSC04010.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The spinach leaves ready for light treamtment.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiSLX0Zq8IphJxSaS8VTQVBTqCq9u1kld3ptc2AtBA3vnxyq_Lo6kw9dv462FRN4v0-MZ8IPT1TGCgPv4uz83NLS8fJDMBwDHkdN9SXhDsfUSVEAXgJzZRLvrGFS8WNvPPweQt3RC8zq4Ks/s1600/DSC04011.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiSLX0Zq8IphJxSaS8VTQVBTqCq9u1kld3ptc2AtBA3vnxyq_Lo6kw9dv462FRN4v0-MZ8IPT1TGCgPv4uz83NLS8fJDMBwDHkdN9SXhDsfUSVEAXgJzZRLvrGFS8WNvPPweQt3RC8zq4Ks/s1600/DSC04011.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The spinach leaves covers in cling film and starting light treatment.</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhNxPNjukgpRkZBa5rtExEjNTGiPVvtfoZO02h_GlxIry3nnLeaJYexN-mhbMIwzdty00EbALNpC7GM4rsQ0pyPoCfIt2hrfFGr9z86A-Tw2Oc-ESom30L_mHgtRuPx2tnor0NMXiQuuFP6/s1600/DSC04012.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhNxPNjukgpRkZBa5rtExEjNTGiPVvtfoZO02h_GlxIry3nnLeaJYexN-mhbMIwzdty00EbALNpC7GM4rsQ0pyPoCfIt2hrfFGr9z86A-Tw2Oc-ESom30L_mHgtRuPx2tnor0NMXiQuuFP6/s1600/DSC04012.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Measuring the light intensity for the light treatment~ it was between 450-500 micro Eisenstein. This device is so cool it like an botanist stethoscope, haha!</td></tr>
</tbody></table>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhXEadYlmpoYwZajUvoylo2vIvHMSJAQYu6SFulf-Yt-j1qCXi1ag-81HgDu4iOCTOYM-mQAs5a5vb8K8c7-SCgovRn5xST59cqPdqa2mgYg-PQv3k3fQN7kjQ1jOr3ipxi5KTeWj5Bug7Z/s1600/DSC04013.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhXEadYlmpoYwZajUvoylo2vIvHMSJAQYu6SFulf-Yt-j1qCXi1ag-81HgDu4iOCTOYM-mQAs5a5vb8K8c7-SCgovRn5xST59cqPdqa2mgYg-PQv3k3fQN7kjQ1jOr3ipxi5KTeWj5Bug7Z/s1600/DSC04013.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Further measuring of light intensity and you can see the other plants grown by other researches at Queen Mary, in the plant room :)</td></tr>
</tbody></table>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiMmdjFdzxXvYVT433OZqXbHDgjje5-tuHax3080pepFKLKXwgPKwviBgN4ZRJlr6XSejM6ABLb65mEPJnkVNnmAY-jqJeZoFXwG-8TtWZRGkvidZrCd3pny5u_ZBl_oMyb0dafwPO0L4rS/s1600/DSC04014.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiMmdjFdzxXvYVT433OZqXbHDgjje5-tuHax3080pepFKLKXwgPKwviBgN4ZRJlr6XSejM6ABLb65mEPJnkVNnmAY-jqJeZoFXwG-8TtWZRGkvidZrCd3pny5u_ZBl_oMyb0dafwPO0L4rS/s1600/DSC04014.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Reading through the instructions and adding extra notes for next time.</td></tr>
</tbody></table>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjXUXGkwjZFgRhxQ0RHrzGvWDxXQK4UAgvroohUMS1BH78H6znpNRflYb68ZCKxL60FkDC7fZJR8XetdKClFxwW-MjqCUS1W0Do8Og-LvEzWbN6Ig3ddDx4y0U5ataHVkvIqYD_65vhe1nr/s1600/DSC04015.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjXUXGkwjZFgRhxQ0RHrzGvWDxXQK4UAgvroohUMS1BH78H6znpNRflYb68ZCKxL60FkDC7fZJR8XetdKClFxwW-MjqCUS1W0Do8Og-LvEzWbN6Ig3ddDx4y0U5ataHVkvIqYD_65vhe1nr/s1600/DSC04015.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">While waiting for the centrifuge cycle, preparing of the buffers and mediums ready to add to the pellet of the centrifuge tubes.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiy_-XrDbk_oyBj1bMdTCvT5nB0UwfNpOa4VeC15VD2kQ26D1Udg54ilO6y1ABoRlcgHSwg3qKepkn-Snx5fGXiIK2IxeKm2kgd3fGUxX65MkXvQPCU2jflYNi2d0kja9u4e8uS91h6zn7w/s1600/DSC04016.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiy_-XrDbk_oyBj1bMdTCvT5nB0UwfNpOa4VeC15VD2kQ26D1Udg54ilO6y1ABoRlcgHSwg3qKepkn-Snx5fGXiIK2IxeKm2kgd3fGUxX65MkXvQPCU2jflYNi2d0kja9u4e8uS91h6zn7w/s1600/DSC04016.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The osmotic medium added after the break medium which breaks the photosynthesis membrane into smaller pieces, the break medium is added for 30 seconds, before the osmotic medium is added.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi3eimypVZ1hMEjVFJYfyHejbzYlN4b4qD0djLZNCKGtKxjCXq0FlTVx8mBDET9X9VGUHadJhw4xCbzmTC13ZbhHmDUm0_HiuTpJBAqfuq_i2TIDfLZbOphMgwOC3KnFpFwS8EffM7Bkqd3/s1600/DSC04017.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi3eimypVZ1hMEjVFJYfyHejbzYlN4b4qD0djLZNCKGtKxjCXq0FlTVx8mBDET9X9VGUHadJhw4xCbzmTC13ZbhHmDUm0_HiuTpJBAqfuq_i2TIDfLZbOphMgwOC3KnFpFwS8EffM7Bkqd3/s1600/DSC04017.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">A centrifuge! You always learn about them throughout education in science. I always never really understood the true importance of them, till i actually used them in this studentship. They are such amazing devices and I've learnt to absolutely love this one in Ruban's lab! :D</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgTzQO2SAKKVWEJwwyTfQKm_P_-4CvUzpAeUGOtVZ1-8su5RqT1oaIb_uY4cdZS1aqGyJYbFg_3g5EIWUgp75s94fZiUhS0LlcABL74H_xpYiy7pKGmGutQK-1ZjpxwRbuHjVniMj8OskY2/s1600/DSC04018.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgTzQO2SAKKVWEJwwyTfQKm_P_-4CvUzpAeUGOtVZ1-8su5RqT1oaIb_uY4cdZS1aqGyJYbFg_3g5EIWUgp75s94fZiUhS0LlcABL74H_xpYiy7pKGmGutQK-1ZjpxwRbuHjVniMj8OskY2/s1600/DSC04018.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Centrifuge walkthrough, left to right- Time: 1.41 seconds remaining of the spin, Rotations per minute: 4000, Temperature: 4 degrees and not sure about what the 9 means, think its pre-set!</td></tr>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEimjsB0w5aKVEkloMN9mHpk7V1ZyE33oYa9wgcoceOS9F1y1lXLFA4FAbsAxdQLzFKhIeVrJXM6SdDAC_8bNRkeKbPCtpiYcuqXAMh-Fd0REWH5gYukf-uy7R-hyF2vG_MOngJN4lM39jYY/s1600/DSC04021.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEimjsB0w5aKVEkloMN9mHpk7V1ZyE33oYa9wgcoceOS9F1y1lXLFA4FAbsAxdQLzFKhIeVrJXM6SdDAC_8bNRkeKbPCtpiYcuqXAMh-Fd0REWH5gYukf-uy7R-hyF2vG_MOngJN4lM39jYY/s1600/DSC04021.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Love my university lab coats, how they embroided the logo on every single one. Luckily for me X-smaller is in the colour of red and i love the colour red :) It's chinese lucky colour! However, if I'm honest pink is my favourite, it just makes me feel so happy and girlie, hahah...</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjGZVfYP9WdOtc_MNKLgMpMdsOahuu5oEZ2HbA9QvTgDllZ7w7krpS9XFdJ4XmceKsqXbHJTLdzK4SbS25IYjSLxBRuycb77PDBWAhtxexYBmanLuO-PAHZfH0duZsRU268OHJoaRtRZQEg/s1600/DSC04022.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjGZVfYP9WdOtc_MNKLgMpMdsOahuu5oEZ2HbA9QvTgDllZ7w7krpS9XFdJ4XmceKsqXbHJTLdzK4SbS25IYjSLxBRuycb77PDBWAhtxexYBmanLuO-PAHZfH0duZsRU268OHJoaRtRZQEg/s1600/DSC04022.JPG" height="320" width="213" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Taking buffer from the FPLC machine~which we need for further steps in the instructions.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhb7rwBdr8wIe3GeMfeMKqQnFGHiWwzu3SeRo1i59bVbrmtmO4Cg1s00PlrpSNPGf22V9FTJbpIrP1P8lUECEP1NTt6Jz-ZtxP3CKNjZy-Y-U4pNkknbdNVLk68jY1mLwFxzJTDwe15y179/s1600/DSC04023.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhb7rwBdr8wIe3GeMfeMKqQnFGHiWwzu3SeRo1i59bVbrmtmO4Cg1s00PlrpSNPGf22V9FTJbpIrP1P8lUECEP1NTt6Jz-ZtxP3CKNjZy-Y-U4pNkknbdNVLk68jY1mLwFxzJTDwe15y179/s1600/DSC04023.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Other picture of the FPLC machine, looks pretty cool~ overwhelming at first.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgfpGKPyASDlRmm3Q1PFOOtDIOWlaeQ50m2PvBN-p8esOVDwWXrNRvsxEjXFdrZPyxTx5UPr9r68MWxT7RuIX6orHpn2YIQVdK8aQ4pc3fnzoNddz481M2C_sNRLuDD-Azv2c8TuW0uzvKS/s1600/DSC04025.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgfpGKPyASDlRmm3Q1PFOOtDIOWlaeQ50m2PvBN-p8esOVDwWXrNRvsxEjXFdrZPyxTx5UPr9r68MWxT7RuIX6orHpn2YIQVdK8aQ4pc3fnzoNddz481M2C_sNRLuDD-Azv2c8TuW0uzvKS/s1600/DSC04025.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="text-align: start;">So basically watching the different separation of fractions in the FPLC on the PC screen. The different wavelength of lights indicate what is observed. As we know wavelength of 670</span><span style="background-color: white; color: #252525; font-family: sans-serif; font-size: 14px; line-height: 14.933333396911621px; text-align: start;">λ</span><span style="text-align: start;"> are of chorophyll A, 480</span><span style="background-color: white; color: #252525; font-family: sans-serif; font-size: 14px; line-height: 14.933333396911621px; text-align: start;">λ</span><span style="text-align: start;"> is for both chorophyll A and B and pigments, 280</span><span style="background-color: white; color: #252525; font-family: sans-serif; font-size: 14px; line-height: 14.933333396911621px; text-align: start;">λ</span><span style="text-align: start;"> gives proteins</span><span style="color: #252525; font-family: sans-serif; text-align: start;"><span style="background-color: white; font-size: 14px; line-height: 14.933333396911621px;">.</span></span></td></tr>
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So by the end of the first full day in lab I have completed pretty much nearly all the steps in the protocol plan for the 8 weeks! I was initially worried if I would have enough time in the 8 weeks to do it all, as I didn't know anything about how long each step will take. However, after today I understand the whole project alot more! That 8 weeks is plenty time to get really nice results in this project. That mainly the 8 weeks will be analysing the fractions that I obtain from solubilisation, to find the efficiency of Violaxanthin de-epoxidation in PSII supercomplex. </div>
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<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgXNwutnHSQoD0AMHRzhxpfQjR_cRDc4NpzCHBYPmaLPgSdWnL3d9WVp2GDpDokcP80zWxmEO9zj4K_xzHdUjopXaV1g3bvWhBRcZ0G56nCozixhoBVx8ypGR-Wrp3bDC0jhIvzacTmZkDy/s1600/protocol.png" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgXNwutnHSQoD0AMHRzhxpfQjR_cRDc4NpzCHBYPmaLPgSdWnL3d9WVp2GDpDokcP80zWxmEO9zj4K_xzHdUjopXaV1g3bvWhBRcZ0G56nCozixhoBVx8ypGR-Wrp3bDC0jhIvzacTmZkDy/s1600/protocol.png" height="245" width="400" /></a></div>
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I am quite excited to move onto outline step B. I always think working with mutants is interesting as they can clearly indicate the purpose of certain complexes in each organisms.</div>
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Tomorrow I shall be analysing the results obtained from FPLCJ.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-25139736568463233602014-05-26T19:00:00.000-07:002014-06-08T06:59:51.261-07:00Bank Holiday Monday: Back in London! <span style="text-align: center;">Bank holidays are always fun, or relaxing at least </span><span style="font-family: Wingdings; text-align: center;">J</span><br />
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So today, me and my family went to a Chinese restaurant to eat and then had
casual shop around Sheffield town centre, before I got on the train back to
London! In ways it is always sad departing from loved ones, but London just
offers more excitement for my outgoing nature so I’m always happy to go back. I
think if I could drive, Sheffield would be a fun place too as I could visit all
my friends easily, but London has the fast connection of the tube, so I can get
anywhere practically <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;"><span style="mso-char-type: symbol; mso-symbol-font-family: Wingdings;">J</span></span>
Plus the boyfriend to drive you around is a bonus as well ^_^<br />
<br />
Anyways~ finally back and its around 7pm, I’ve started reading up on the
chapters that my supervisor suggested. It’s really cool as he has written a
book which is so condense and references many papers, it makes his research
area sort of like a walk through. <o:p></o:p></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgwiH5c8eoynLcryGVREiFrAvKMo2B7UXWS37ob6BS_xJfyfthQao3qYbYMXKJSRLzbwsrhH-tqB_PoHczYdVHi_JqCv7hpBDZAKPKxRLXY3Eouuh-kcerDvw2N7vhQyBf5W7yyYW7ahQzj/s1600/DSC03914.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto; text-align: center;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgwiH5c8eoynLcryGVREiFrAvKMo2B7UXWS37ob6BS_xJfyfthQao3qYbYMXKJSRLzbwsrhH-tqB_PoHczYdVHi_JqCv7hpBDZAKPKxRLXY3Eouuh-kcerDvw2N7vhQyBf5W7yyYW7ahQzj/s1600/DSC03914.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Ruban's photosynthetic membrane book, just making notes on each page to help me further understand. Alot of the time in science there are words which confuse me so I always need to find out the definition or action of a certain pigment I'm reading about. I like to have the generalise view.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjpAOKmVOaubCbwfKTfX6Cv_6lfh29XeI7RGEDNC15CD8_U9Zr8qLBaoaNYXgxD8XRPqmbHSNvLf6fQNhm75iXnt5xj0-lktrWClpe5UeU-Dsm3KSF3RSnzoojC0cK84LVewomFrMnemFC3/s1600/DSC03918.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjpAOKmVOaubCbwfKTfX6Cv_6lfh29XeI7RGEDNC15CD8_U9Zr8qLBaoaNYXgxD8XRPqmbHSNvLf6fQNhm75iXnt5xj0-lktrWClpe5UeU-Dsm3KSF3RSnzoojC0cK84LVewomFrMnemFC3/s1600/DSC03918.JPG" height="193" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="font-size: small; text-align: start;">This is what my supervisor sent me, it's basically what I will be doing for the 8 weeks! OOOH excitment ^_^</span></td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiQT5gvUonmXR1kx_-EF8iCOgdOwC3R3GO0nku7txJgMm0PFDKmyOS8AhL5HX9ExUf_QRTf-FuGvD2M8AI-Wf_jqorUUiZZ8QQaM9wconf-XGAKAuPgrtecRyKTff2YRkG4rDX1Kyz6sftX/s1600/DSC03915.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto; text-align: center;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiQT5gvUonmXR1kx_-EF8iCOgdOwC3R3GO0nku7txJgMm0PFDKmyOS8AhL5HX9ExUf_QRTf-FuGvD2M8AI-Wf_jqorUUiZZ8QQaM9wconf-XGAKAuPgrtecRyKTff2YRkG4rDX1Kyz6sftX/s1600/DSC03915.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Here is the experimental protocol, reading up on it so I know what I need to do tomorrow! I hate the feeling of not knowing the next share and uncertainty when following instructions for an experiment. Need to know everything before hand!</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjo7nhzJL7_J48n39RxxaArXW426_JjBRqdmIawLoIyZpXWbo6ndhSRMIQqtOWaiU2kymKdUCAwz-GuzgQAS8bR1MHCBdzVXKer9gKUp4MliPWfJCoigcfi0tAdP88vG7NZSwGxpdgiMgGN/s1600/DSC03916.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto; text-align: center;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjo7nhzJL7_J48n39RxxaArXW426_JjBRqdmIawLoIyZpXWbo6ndhSRMIQqtOWaiU2kymKdUCAwz-GuzgQAS8bR1MHCBdzVXKer9gKUp4MliPWfJCoigcfi0tAdP88vG7NZSwGxpdgiMgGN/s1600/DSC03916.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="font-size: small; text-align: start;">My first year lab book, we did a lot of practicals in first year and I wrote alot of notes on how to use micro pipettes, centrifuges and do calculations. All which are essential lab skills ^_^</span></td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj1_4Y4q2DvwMtDf7-6sC42J1R_LjlLWGexDb11-uD83QmNkNDW7RpHrcPRM1keiy2oWR9MqxZZdqA-vrb4ceySG97rRB87cEktfzLcVTuoX1MJMHs0CCBlrmhJDNcCQtHyeiasIHgRAw0u/s1600/DSC03909.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto; text-align: center;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj1_4Y4q2DvwMtDf7-6sC42J1R_LjlLWGexDb11-uD83QmNkNDW7RpHrcPRM1keiy2oWR9MqxZZdqA-vrb4ceySG97rRB87cEktfzLcVTuoX1MJMHs0CCBlrmhJDNcCQtHyeiasIHgRAw0u/s1600/DSC03909.JPG" height="213" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="font-size: small; text-align: start;">My amazing corner study desk~ where all the geeking out happens :D Really into spacing saving designs, so this corner desk is just amazing !</span></td></tr>
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Although, because I am mainly a Medical Geneticist, and my
degree consist more of biology/ biomedical related modules than biochemistry,
this research project I will have to read up extra on, as it is in a different
field to mine. To be honest I was really considering to do biochemistry, as I
enjoy remembering the pathways, different mechanisms…I love learning pattern.
Lots of people told me to not do biochemistry because it’s boring and it’s all
about remember all the long amino acids name and structures, so I didn’t.
Little did I realise that when I did do my biochemistry modules in first year,
I actually loved all the shizzle that people said were boring. Life lesson
learnt never listen to others and follow your heart <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;"><span style="mso-char-type: symbol; mso-symbol-font-family: Wingdings;">J</span></span> In ways Medical geneticist
offered a better degree in a sense if I decided to do medical research (which I
do quite enjoy reading up on) or decide for graduate entry for
Dentistry/Medicine/ Veterinary… it’s just good to keep options open, uncase I
do change or discover my new passions for areas <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;"><span style="mso-char-type: symbol; mso-symbol-font-family: Wingdings;">J</span></span>
Also, genetics is the fundamental science of life really, it what makes us all
unique, so I do enjoy it!<o:p></o:p></div>
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Yeah, so I’ve been basically reading up on the chapters and
relevant papers he has suggested. Although, personally I find it quite hard to
see how they are relevant. Tomorrow is my first day in the lab so I’ll see how
it all shall link. For me I always believe it’s easier to see how things relate
in content than reading in book. Once I can see the link it all goes in and it’s
easier to logically think about~<br />
<br />
So why everyone is having those bank holiday Monday raves! I’m inside reading all
this, it’s like exams all over again! Hahah, joke really. This is nothing like
exams as there is no pressure of actually sitting an exam. I actually really
enjoy learning, especially because this is area which is different to my actual
degree. I’ve never learnt about photosynthesis in this depth before <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;"><span style="mso-char-type: symbol; mso-symbol-font-family: Wingdings;">J</span></span> People say to me omg
you don’t get a break after exams, which is crazy! But I prefer life to be like
this. I don’t really like sitting in my room all day and doing nothing. I
actually like doing something, a mix of things, variety. My studentship was actually
meant to start 2 weeks after my final exam but I decide to pull it forward to
straight after my exams! It’s better to get started early, especially because I
am eager. Also, I have a third year project to start as well after my
studentship, on something different maybe… Ruban said we can decide that later
on anways~<o:p></o:p></div>
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Super excited for tomorrow <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;">J</span>
Even though it’s an 9am start and that involves me going underground for
30minutes, which is quite depressing in the mornings, but I’ve gotten use to it
now I’ve been in London for 2 years ! <o:p></o:p></div>
J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-82112462927542440922014-05-24T10:00:00.000-07:002014-06-08T06:34:40.550-07:00The weekend of end of exams!<div class="Publishwithline">
So basically it’s the weekend of the end of my exams! Woo!
Crazy how I’ve taken 8 exams this summer, so glad that next year we only have
to take 6, due to the third year project being worth 30 credits ^_^</div>
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Usually, people would be going to go clubbing to celebrate,
but not all my friends have actually finished their exams, especially my closer
friends from different universities. I guess one of perks of being in my course
is that we always finish really early, compared to other universities in
London/ around the U.K. Anyways, I decided to go home to visit my family, it’s
been a while. I don’t always go back every week like a lot of students do.
Sheffield is 2hours away from London on the train, also I always have something
to do in London. This is the reason why I decided to come to London for
University in the first place. It is the city that never seems to sleep! (I
know everyone says that about New York too, but I think it’s true for London)
There is always something to do in London, something new and exciting. It’s
such a big city therefore a lot of areas and events are always going on. I
never get bored! Also, it is so culturally enriched and I love being exposed to
vast cultures- it just opens your mind more to the beautiful world we have <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;"><span style="mso-char-type: symbol; mso-symbol-font-family: Wingdings;">J</span></span><o:p></o:p></div>
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So walking around Sheffield town centre with the family
looking for something to give to my blog and I see a plant stores outside the
town hall. Here are some pictures.</div>
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<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhrOwYPDgsApQ-cVlSMzx6qctCnRTJnYDSri0pLsGpOkeUs3VHC4oHB9kVz34jHBLKqC2aD0CJXcsAP4NvLqZ9o_McsABZP-9BVRJAMfChdgZt9d6KdO7BsF0qeqQexw8FpVVZ4qpQluJPv/s1600/DSC03902.JPG" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhrOwYPDgsApQ-cVlSMzx6qctCnRTJnYDSri0pLsGpOkeUs3VHC4oHB9kVz34jHBLKqC2aD0CJXcsAP4NvLqZ9o_McsABZP-9BVRJAMfChdgZt9d6KdO7BsF0qeqQexw8FpVVZ4qpQluJPv/s1600/DSC03902.JPG" height="213" width="320" /></a></div>
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<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi8ho4VAQmwJw362oRLwX56I3N2yA_NQGztxx7EXpWEhHoOJ2PWidjHlSQvYAGgXE0rWbAlCl1MKhqDmNcW1QInwSjBM1_u_fjRMxjqq5G9_X4JSwfwXjw5wKn-PnpkIuEV_e0fwMMQZCNr/s1600/DSC03906.JPG" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi8ho4VAQmwJw362oRLwX56I3N2yA_NQGztxx7EXpWEhHoOJ2PWidjHlSQvYAGgXE0rWbAlCl1MKhqDmNcW1QInwSjBM1_u_fjRMxjqq5G9_X4JSwfwXjw5wKn-PnpkIuEV_e0fwMMQZCNr/s1600/DSC03906.JPG" height="213" width="320" /></a></div>
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<br />
This actually got me really excited for the start of my studentship on Tuesday
(Monday is a bank holiday, woo woo! Don’t we all love bank holidays?! :D) I’ve
always thought plants were beautiful organisms, in ways seeing a plant is quite
refreshing, especially because in London there is little greeny which surrounds
me. I have always been interested in photosynthesis, its such a complex
mechanism, I know at the time learning at A-levels it being very complex, when
actually when you think back the way they thought it in A levels is so simple
compared to what it is really….Anyways, I really like photosynthesis, it’s a really
neat process, it basically is the reason how we and practically all of the
other organisms evolved on life. We wouldn’t even be here if it wasn’t for
plants. So it’s quite sad how people just overlook them and not really realised
their importance.<br />
<br />
I was recently reading a nature review about why we need plant sciences, as
they are the scientist which would unlock the answers to. How will we feed the children
of the future? How can plants contribute to the energy crisis? It’s true that
this is an area in science that is often neglected. I don’t think that the UK
education system really implements plant science careers into the minds of
young children. Many aspire to be lawyers, doctors, football players, police
men, business owners, but rarely does anyone actually want to be a plant
scientist. To be honest I’ve always been interested in Medical sciences, hence
the reason why I’m undertaking a degree in medical genetics, but it just hit me
recently, what is the point in saving so many people dying from disease, if we
just simply do not have the food and energy supply to aid their lifestyles? I
like to think about the future generations, some people say global warming is
just a natural phenomenon, but I truly believe because of extra carbon
emissions in the world, we have severely increased the global temperatures and
sea levels, causing devastating effects to the world- such as freak weather and
the extinction of animals. I want children in the future and I’m sure the cycle
of them wanting children will continue for generations. I want the human race
to survive and not be wiped out because we were foolish with damaging the world
generations before. Hence, one of the reasons why I’m undertaking a project in
photosynthesis, particularly relating to photosystem 2 and the pigments related
in light stress. If I can understand the fundamental mechanisms behind photosynthesis,
this will aid me in my future research plans in artificial photosynthesis or
generations of biofuel energy by genetic modification of plants? <o:p></o:p></div>
J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0tag:blogger.com,1999:blog-4371744833186781390.post-9666417301453990792014-05-23T10:00:00.000-07:002014-06-08T06:42:16.226-07:00First blog, little introduction to photosyn-this! <div class="MsoNormal">
So here it is, my first blog <span style="font-family: Wingdings; mso-ascii-font-family: Calibri; mso-ascii-theme-font: minor-latin; mso-char-type: symbol; mso-hansi-font-family: Calibri; mso-hansi-theme-font: minor-latin; mso-symbol-font-family: Wingdings;">J</span>
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In this blog I will mainly be talking about my feelings
towards the whole studentship and science issues which are relatable to my
areas of interest in research. The two main themes will be upon artificial
photosynthesis & synthetic biology. I really feel that synthetic biology is
the way forward in life! I will try and write it in a simplistic way so even
non-scientific aware readers can easily pick up on this!<o:p></o:p></div>
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Anyways, let’s start
from the beginning. I remember when I got my acceptance letter for the
Biochemical Society Studentship. It was during the start of my exam season,
which is never fun. However, I did remember being very joyous at the time. Not
trying to be arrogant or egotistic in anyways, but I kind of always knew I
would get it, not because I am a role student, but because I really put my
heart into writing the whole personal statement for this studentship. I truly
believe if you put your entire soul and heart into something, the outcome
should always be positive, unless it was never meant to be.<br />
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiRIcxBAUoNpdDGa8i3EnWqks5zZWGYIrc3uKHG_UAzTJ2igeHP_IIBHhAOHims2HgO3nIuq50FLFicWOKVCrRZxfaIT5ia_mdHLsfQM7eZ69CAX_EkTNJY9d0lMPAy_XuNb4oAyLGLJVt3/s1600/Capture.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiRIcxBAUoNpdDGa8i3EnWqks5zZWGYIrc3uKHG_UAzTJ2igeHP_IIBHhAOHims2HgO3nIuq50FLFicWOKVCrRZxfaIT5ia_mdHLsfQM7eZ69CAX_EkTNJY9d0lMPAy_XuNb4oAyLGLJVt3/s1600/Capture.JPG" height="203" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The overwhelming happiness that came from this email, WOOHOO!! I GOT IT :D</td></tr>
</tbody></table>
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The whole reason why I applied for the studentship was
because while searching for options after graduation and generally reading
about science newspapers/ books. I stumbled upon a book called “Regenesis: How synthetic
biology will reinvent ourselves and nature.” It was from reading this book that
I discovered my true inner interests in synthetic biology. I finally found
something that was innovative enough and a science that could really offer big change
in the world. I’ve always been really into the whole “energy and sustainable
living” themes in life. I think it arose from GCSE geography. I think I just
appreciate energy because I know without it I would not have the lifestyle that
I enjoy to live, such as being able to Whatsapp my friends and use the internet
to read upcoming news, being able to travel to other countries and explore-
something I love so dearly. So basically after I just searched in my school
department for a professor perfectly suited to my future goals in life, and I
found him. Professor Alexander Ruban. Gosh, wasn’t I lucky to find him, he is
such an expertise in his field and regularly publishes papers *o*. Obviously at
the time I didn’t know this, but once I knocked on his door I knew he was the supervisor
to pick because he was so passionate about his research and it seemed like he
would be helpful if I ever got confused with the studentship. Call me lucky, as
he was the first one I approached and was so willing to take me on. Maybe some
things were destined? Ha.<o:p></o:p></div>
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<br />
In ways I believe I am an activist in character, I want change. I want to see
change in this world. I think ever since University and the whole maturity and
aging process, I have stopped turning a blind eye to these modern day issues,
which no one really actually genuinely worries/ cares about. Seriously, ask
your friends or family members, when do they ever think about how the energy
resources are rapidly depleting in this world? How would such depletion affect
the way the modern day world turns? You won’t have your laptops, I-phones or
cars….we would basically revolt back to the stone ages (I know that such
happenings are very unlikely to happen, but it could!) Things that I really
think need to be tackled in the energy problem. Yes, I know there are many
researchers already working on this issue, but I think the 21<sup>st</sup>
century, energy really should be in the form of renewable, nontoxic and non-harmful
source. This is why I think artificial photosynthesis is the way forward,
already there has been a developing prototype of a “leaf” which can use the
sunlight, to convert CO₂ and H₂O into O₂ and H₂, which H₂ can be used for
fission to create energy. It is eventually hoped that the “leaf” will be stored
in homes, being able to generate energy for the entire household. So how cool
is that? Having a device which acts like a leaf attached to your house,
generating energy spontaneously when there is sunlight, then storing it for
night time use? You would never even need to pay an electricity bill ever again
as you are basically taking energy directly from the no.1 source in the world:
the potent sun! Think how much money you could save and how much better the
world would be, because we would no longer need pylons for the national grid…
Obviously, the leaf device itself will not be cheap, but like most good things
in life, investments need to be made in order to get good returns. It is true
for anything in life.<o:p></o:p><br />
<br />
Anyways, I shall end with nice, easy to watch videos about artifical photosynthesis. I love watching educational videos like these on youtube. For me it's always easier to learn when watching a video than just plain reading~ I'm a visual learner :)<br />
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<iframe allowfullscreen="" frameborder="0" height="270" src="//www.youtube.com/embed/J556uXwrjII" width="480"></iframe>
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Here is the artificial photosynthetic leaf that I've been on about in this blog. Love it! Think if most of you watch this video you will probably understand my enthusiasm for this area of research. It's just so exciting :) Alot of my friends who I tell them about this, get a really wow reaction too!</div>
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This is another one of my favourite videos, which made me really get into artificial photosynthesis. When I find a research area that I like I always youtube it first for a overall easy overview on the topic! This video clearly highlights the fact that artificial photosynthesis could help reduce carbon emissions and global warming; hence a probably decrease in freak weather, while also generating energy! Also researchers are from Panasonic! Find it really cool how Panasonic are investing into this research :) Having being to Japan myself and absolutely love their culture, it's cool to know that a country I love so much I could possible work in the future, if I plan to specialise into artificial photosynthesis. Especially, puts my Japanese skills that I've been learning at night class over my entire 2nd year to use :)</div>
J.Lauhttp://www.blogger.com/profile/03643017650571532406noreply@blogger.com0